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On March 9, 2023, a Cabinet Office Ordinance partially amending the food labeling standards was promulgated, adding "walnuts" to the list of specified ingredients, making it mandatory to label allergens, which now include eight items: "shrimp, crab, walnuts, wheat, buckwheat, eggs, milk, and peanuts." In this seminar, we will introduce the qualitative real-time PCR method for walnuts (Walnut-H method) in accordance with the Consumer Affairs Agency notification, as well as the qualitative real-time PCR methods for wheat, buckwheat, and peanuts that were simultaneously included with this amendment, along with inspection techniques. Co-hosted by: House Foods Group Headquarters Co., Ltd.