極東製薬工業 List of Products and Services

We are looking for individuals who can evaluate samples for product commercialization. (1) It is a pepton derived from soybean protein digested by microbial enzymes. (2) Being a plant-based pepton, it avoids the risk of contamination from bovine spongiform encephalopathy (BSE) and animal-derived viruses. (3) It is rich in carbohydrates and vitamins, demonstrating high growth support for the culture of general bacteria and fermentation industries.

• Other bioproduct raw materials
• Yeast and fungi

This product is a medium for the suspension culture of human fetal kidney cell-derived 293 cells (e.g., Expi293F cells, 293T cells, etc.). It can be used for the expression and production of recombinant proteins utilizing 293 cells. It allows for stable subculture and achieves high transfection efficiency. 【Features】 - Manufactured in our domestic factory (Made in Japan) - Transfection using lipofection or polyethyleneimine methods is possible - Serum-free medium with a clearly defined chemical composition - Free from animal-derived components and proteins

• Culture medium
• Other bio-related products
• Other cell research

This catalog presents a comprehensive introduction to cell-related reagents, including culture media for cell culture, cell preservation solutions, dispersing solutions, and products related to iPS/ES cell culture. In addition to product introductions, it is packed with tips for product selection, such as "Key Points for Choosing Media for iPS Cells," "Key Points for Choosing Dispersing Solutions," and "Key Points for Choosing Freezing Preservation Solutions!" It also includes numerous usage flows, experimental schemes, and application examples. *The materials can be downloaded via the button below.*

• Other bio-related products and services
• reagent

"CP-1 High Grade" is a cryoprotectant solution that allows for the cryopreservation of cells such as hematopoietic stem cells and mesenchymal stem cells at -80°C without the use of a programmable freezer. Since it can be cryopreserved without managing the cooling rate, it reduces the time and effort required when freezing large quantities of cells at once. It does not contain animal-derived materials, has confirmed suitability for regenerative medicine product materials, and has undergone non-clinical testing, making it a product with excellent quality and safety. 【Features】 ■ Registered in the Master File (MF) for active pharmaceutical ingredients ■ Manufactured at our GMP-compliant facility in Japan ■ Uses USP-grade DMSO ■ Conducted sterility tests, endotoxin tests, and mycoplasma negative tests ★ You can view the document "Cryopreservation of Human iPS Cells" using evaluation data from the Kinooka Laboratory at Osaka University via "PDF Download." Please feel free to contact us for sample evaluation inquiries.

• others

In this seminar, we will hear a lecture from Dr. Katsuhisa Matsuura on the basic concepts of iPS cells, as well as the large-scale culture technology of iPS cells he is working on and its application research in cardiac tissue engineering. ◎Title "Development of Large-Scale Culture Technology for iPS Cells and Its Application in Cardiac Tissue Engineering" ◎Speaker Dr. Katsuhisa Matsuura (Tokyo Women's Medical University, Advanced Research Institute for Life Science) ◎Date and Time July 11, 2022 (Monday) 10:00 AM to August 8, 2022 (Monday) 1:00 PM ◎Target Audience Individuals involved in research, development, manufacturing, planning, or sales in the field of regenerative medicine/cell engineering *To apply for viewing this seminar, please proceed to the application form via the link below.

• Technical Seminar

Plexin domain containing protein 2 (PLXDC2), a cell surface transmembrane protein, is expressed in many tissues including haemotopoeitc stem cells, neural stem cells, pluripotent stem cells, and tumor cells. PLXDC2 may play an important role in neuronal growth, stem cell development, angiogenesis, and cancer cell growth. Recently PLXDC2 was reported a good cell surface marker as human haemotopoetic stem cells

• Research Antibodies
• Flow cytometer
• Other contract manufacturing

Kyoto Pharmaceutical Industry has entered into an exclusive sales agreement for innovative antibody development and antibody engineering services provided by Abwiz Bio Inc. in the United States, and is supporting Japanese customers as the sole agent in Japan. Here, we introduce recent achievements in acquired antibodies. If you are considering contract services for antibodies, please make use of this information. [Contents] ◎ Production of rabbit monoclonal antibodies for GPCR and ion channels ◎ Detection of multidrug-resistant bacteria (anti-CTX-M rabbit monoclonal antibody) ◎ Immunohistochemical staining of cancer using anti-PLXDC2 rabbit monoclonal antibody ◎ Improvement of affinity for mouse monoclonal antibodies against antigen X *This document can be viewed via "PDF Download." Please feel free to contact us with any inquiries.

• others
• Contract manufacturing
• Other polymer materials

In this seminar, Dr. Yamahara will give a lecture on the commercialization of mesenchymal stem cells he has been working on, as well as the CP-1 used for their cryopreservation. ◎Title 　'Cryopreservation of Clinical Mesenchymal Stem Cells Using CP-1' ◎Speaker 　Dr. Kenichi Yamahara (Hyogo College of Medicine, Advanced Medical Research Institute) ◎Date and Time 　March 9, 2022 (Wednesday) 10:00 AM - April 8, 2022 (Friday) 1:00 PM ◎Target Audience 　Individuals involved in research, development, manufacturing, planning, or sales in the field of regenerative medicine/cell therapy. * To apply for viewing this seminar, please proceed to the application form via the link below.

• Technical Seminar

In this seminar, Dr. Makino from Toranomon Hospital will present on the development of the cryopreservation solution CP-1 for hematopoietic stem cell storage, how it is currently used, and what is required for future regenerative medicine, from the perspective of hematopoietic stem cell transplantation. ◎ Title "Development History and Current Use of Cell Cryoprotectant CP-1 High Grade" ~ What is Required for Regenerative Medicine ~ ◎ Speaker Dr. Shigeyoshi Makino (Toranomon Hospital, Blood Transfusion Department) ◎ Date and Time September 27, 2021 (Monday) 13:00 - October 26, 2021 (Tuesday) 12:00 ◎ Target Audience Individuals involved in research, development, manufacturing, planning, and sales in the field of regenerative medicine/cell therapy * To apply for viewing this seminar, please proceed to the application form via the link below.

• Technical Seminar

We are currently offering a "Cell Preservation Solution Selection Guide," which includes descriptions of the features and terminology of each product, allowing you to choose the right product from our lineup of preservation solutions. The guide also includes examples of cryopreservation for various cells, such as mesenchymal stem cells and pluripotent stem cells, as well as a URL to our website showcasing application data for each product. We hope this will assist you in selecting the appropriate preservation solution based on your needs. 【Contents】 ◎ Specifications and terminology explanations of our preservation solutions ◎ A collection of links to pages summarizing cryopreservation examples ◎ An overview of our preservation solutions *This document can be viewed via "PDF Download." Please feel free to contact us with any inquiries.

• others

This antibody specifically detects only the Nucleocapsid Protein (NP) of SARS-CoV-2 and does not cross-react with the Severe Acute Respiratory Syndrome (SARS) coronavirus, the Middle East Respiratory Syndrome (MERS) coronavirus, the four common cold coronaviruses that infect humans (HCoV-OC43, 229E, NL63, HKU1), or other influenza viruses A/B, demonstrating high specificity.

• Research Antibodies

This service is an engineering service for improving antibody performance. It offers various technologies and achievements to accomplish different objectives, such as enhancing reactivity to lower concentrations of antigens, selecting clones with lower affinity, screening considering on-rate or off-rate, and engineering specificity by adding or removing cross-reactivity. 【Features of Our Service】 ■ Meeting customer needs that are difficult to fulfill elsewhere due to our extensive experience and high technical skills ■ Our technicians are Japanese, allowing for thorough discussions in Japanese before work begins ■ The rights to use antibody genes fully belong to the customer under a Fee For Service model ■ Proven track record with major pharmaceutical companies in the United States *Please feel free to contact us for more details.

• Contract manufacturing

The "DD Checker" is a culture medium for the selective detection of various bacteria in food, environment, and bacterial detection. Due to its stamp method, it is easy to operate. The general bacteria version, which uses a color development solution, detects bacteria with high sensitivity and can provide results in as little as 8 hours. It detects invisible bacteria present on cutting boards, containers, hands, cooking utensils, machines, floors, doors, and food. 【Features】 ■ Easy to operate due to the stamp method ■ Selectively detects various bacteria ■ The general bacteria version (using a color development solution) detects bacteria with high sensitivity and can provide results in as little as 8 hours *For more details, please refer to the PDF document or feel free to contact us.

• Culture medium
• Other Culture

CP-1 can easily freeze and preserve various cells. Since there is minimal damage to cells even at -60°C storage, transportation with dry ice is possible! 【Technical Information】 - Examples of cells that can be frozen and preserved using CP-1 Research has also been conducted at -60°C with the assumption of transportation with dry ice. - Literature on the use of CP-1 (partial list) Since its release in 1992, it has over 25 years of proven results, with more than 50 published papers. A partial list of the literature is provided here.

• Other bio-related products and services
• Other research reagents
• Other Culture

"CP-1 High Grade" is a cryoprotectant solution for cells. It allows for the easy cryopreservation of various cells, including mesenchymal stem cells and hematopoietic stem cells. This cryopreservation solution can withstand dry ice transport. 【Features】 ■ Over 25 years of usage since 1992 ■ Numerous published papers ■ Non-clinical trials completed, MF registered ■ Manufactured at our GMP-compliant facility in Japan ■ Can be cryopreserved in a -80°C freezer ■ Composition disclosed, free from animal-derived materials *For more details, please contact us or refer to the catalog.

• Other bio-related products and services
• Other research reagents
• Other Culture

Kyoto Pharmaceutical Industry Co., Ltd. offers antibody services from the American company Abwiz Bio. 【Features of Monoclonal Antibody Production】 ■ Using Abwiz Bio's proprietary antibody gene cloning method, WizAmpTM (patented in the United States), we can produce antibodies with high specificity and high affinity. ■ By combining a vector specialized for rabbit antibody expression with WizAmpTM, known as RabWizTM, we have successfully obtained numerous antibody clones that were difficult to acquire using traditional phage vectors.

• Research Antibodies
• Flow cytometer
• Other contract manufacturing

Kyoto Pharmaceutical Industry Co., Ltd. offers innovative antibody development and antibody engineering services provided by AbwizBio Inc. (Headquarters: California, USA, hereinafter referred to as Abwiz). 【Services Offered】 - Sale of high-quality rabbit monoclonal antibodies - Customized antibody development - Antibody affinity enhancement services

• Research Antibodies
• Flow cytometer
• Other contract manufacturing

An essential item in the field of regenerative medicine: cell preservation solution. We often hear voices saying, "I'm concerned about the specifications and costs, but there are so many options that I don't know how to choose..." This document clearly explains the key points to consider when selecting a cell preservation solution. It has been condensed into five points so that even those who have never been involved in the field of regenerative medicine can read it. *The document can be downloaded via the button below.*

• Other bio-related products and services
• reagent

Kyoto Pharmaceutical Industry Co., Ltd. offers antibody services from the American company Abwiz Bio. 【Antibody Services】 ■ Custom antibody production service (rabbit monoclonal antibodies) ■ Affinity enhancement service ■ Rabbit monoclonal antibody products 【Features of Monoclonal Antibody Production】 ■ Using Abwiz Bio's proprietary antibody gene cloning method WizAmpTM (patented in the U.S.), we can produce antibodies with high specificity and high affinity. ■ By combining a vector specialized for rabbit antibody expression with WizAmpTM, we have successfully obtained numerous antibody clones that were difficult to acquire with conventional phage vectors. 【Features of Affinity Enhancement Service】 ■ Abwiz Bio's unique three-step affinity enhancement platform improves antibody affinity. ■ Achieves over tenfold affinity enhancement. ■ Allows for selection according to various applications through biopanning.

• Research Antibodies
• Other contract manufacturing
• Cosmetic materials and raw materials

"CP-1 High Grade" is a cryoprotectant solution for cells. It allows for the easy cryopreservation of various cells, including mesenchymal stem cells and hematopoietic stem cells. It is a cryopreservation solution that can withstand dry ice transport. 【Features】 ■ Over 25 years of usage experience since 1992 ■ Numerous published papers ■ Non-clinical trials completed, MF registered ■ Manufactured at our GMP-compliant facility in Japan ■ Can be cryopreserved at -80°C ■ Composition disclosed, free of animal-derived materials *For more details, please contact us or refer to the catalog.

• Other bio-related products and services
• reagent

"Kyokuto CHO A2 medium" is a serum-free medium with a clear chemical composition that does not contain proteins or animal-derived components. It has been successfully cultured with CHO-K1, CHO-S, and CHO-DP12. 【Features】 ■ Free of proteins and animal-derived components ■ Does not contain glutamine, phenol red, hypoxanthine, or thymidine ■ Compatible with fed-batch culture and perfusion culture Customization of the medium composition is also available. *For more details, please download the PDF or feel free to contact us.

• Culture medium
• Other contract manufacturing

Abwiz Bio's antibodies are high-quality rabbit monoclonal antibodies. Using our uniquely developed technology "WizAmp" (patented), we select antibodies through phage display methods and produce monoclonal antibodies using recombinant cells. This technology allows us to obtain antibodies with a wide range of diversity that cannot be achieved by other methods. Abwiz Bio's antibodies have high specificity and affinity, and our flagship product, phosphoprotein antibodies, are optimized for the detection of protein phosphorylation in Western blotting and flow cytometry.

• Research Antibodies
• Flow cytometer

We would like to introduce our three main products in the "Pepton and Extracts" category. The pepton derived from milk casein digested with pancreatin, "Far East Pepton (fine granules)," demonstrates high growth-supporting power for the cultivation of general bacteria and for use in fermentation industries. Additionally, we offer "Far East Potato Pepton CP," which is made from potato protein digested with bacterial enzymes, and "Far East Powdered Yeast Extract," which is produced by self-dissolving yeast and drying the soluble components. We also have other products available. 【Main 3 Products】 ■ Far East Pepton (fine granules) ■ Far East Potato Pepton CP ■ Far East Powdered Yeast Extract *For more details, please refer to the PDF document or feel free to contact us.

• Other bio-related products
• Bio-related synthesis and fermentation
• Culture medium

"Kyokuto Pituitary Extract I" is a freeze-dried product of bovine pituitary extract (20mg). By adding it to culture media as a supplement or additive, it promotes the proliferation of various cells. Storage conditions are 2–10°C. The shelf life is 1 year. 【Specifications】 ■ Code: 551-20200-7 ■ Storage conditions: 2–10°C ■ Shelf life: 1 year ■ Packaging: 5 bottles *For more details, please feel free to contact us.

• Supplement materials
• Other additives
• Other cell research

"MTX-LDH" is a kit for cytotoxicity testing using animal cells. It measures LDH (lactate dehydrogenase) released from dead cells. Storage conditions are 2-10°C. The shelf life is 1 year. 【Specifications】 ■ Code: 551-29500-9 ■ Storage conditions: 2-10°C ■ Shelf life: 1 year ■ Packaging: 10 plates *For more details, please feel free to contact us.

• Other research reagents
• Other Culture
• Animal cells

"RD-1" is an additive for "E-RDF medium" suitable for the production of useful substances such as monoclonal antibodies through high-density and large-scale culture. Storage method: Dark place at 2–10°C. Expiration date: 1 year. Contains insulin, transferrin, and ethanolamine. *Toxic substance 【Specifications】 ■ Code: 551-20300-4 ■ Storage method: Dark place at 2–10°C ■ Expiration date: 1 year ■ Packaging: 10 x 1L *For more details, please feel free to contact us.

• Other additives
• Culture medium
• Other cell research

The "Pronase solution for cell dispersion" is used for the dispersion of adherent cells. Storage method: below -15°C. Expiration date: 6 months. This is a made-to-order product, so please inquire about the delivery time. 【Specifications】 ■ Code: 551-28110-1 ■ Storage method: below -15°C ■ Expiration date: 6 months ■ Packaging: 100mL *For more details, please feel free to contact us.

• Cell dispersion reagent
• Other Culture
• Other cell research

"FM-1" is a cryoprotectant solution containing serum-free and protein-free culture medium components. It is suitable for the cryopreservation of serum-free cultured cells. Storage conditions are in a dark, moisture-proof environment at 2–10°C. The shelf life is one year. 【Specifications】 ■ Code: 551-27100-3 ■ Storage conditions: Dark, moisture-proof at 2–10°C ■ Shelf life: 1 year ■ Packaging: 5mL x 5 *For more details, please feel free to contact us.

• Culture medium
• Other bio-related products
• Other cell research

"K-110 Type-II" is a serum-free medium suitable for the culture of normal human epidermal keratinocytes, used for proliferation. (Ca concentration 0.03mM) "K-110 Type-I" is also available for differentiation. (Ca concentration 0.09mM) This is a made-to-order product, so please inquire about the delivery time. 【Specifications】 ■ Code: 551-28204-7 ■ Storage method: Below -15℃ ■ Expiration date: 5 months ■ Packaging: 500mL *For more details, please feel free to contact us.

• Culture medium
• Other bio-related products
• Human cells

"K-110 Type-I" is a serum-free medium suitable for the culture of normal human epidermal keratinocytes, used for differentiation. (Ca concentration 0.09mM) "K-110 Type-II" is also available for proliferation. (Ca concentration 0.03mM) This is a made-to-order product, so please inquire about the delivery time. 【Specifications】 ■ Code: 551-28203-0 ■ Storage method: Below -15℃ ■ Expiration date: 5 months ■ Packaging: 500mL *For more details, please feel free to contact us.

• Culture medium
• Other bio-related products
• Human cells

"Kyokuto E-RDF Medium" is a serum-free medium suitable for the culture of hybridomas. This product is an improved version of RDF (RPMI1640:DMEM:Ham F12 = 2:1:1), with a more enriched composition. It is suitable for the culture of human and mouse hybridomas and myelomas (such as NS-1, MPC-11, MCB-1). It is ideal for the production of useful substances such as monoclonal antibodies through high-density and large-scale culture, and we also offer "Kyokuto Cell Culture Medium Additive RD-1" as an additive. By using a medium supplemented with "Kyokuto Cell Culture Medium Additive RD-1," good growth of hybridomas and long-term large-scale culture at high density can be achieved. "Kyokuto Cell Culture Medium Additive RD-1" contains insulin, transferrin, and ethanolamine. *Toxic substance 【Specifications】 ■ Code: 551-26500-2 ■ Storage method: Dark, moisture-proof 2–10℃ ■ Expiration date: 2 years ■ Packaging: 177g for 10L *Custom media can also be prepared by removing or adding specified substances according to your requirements.

• Culture medium
• Other bio-related products
• Other cell research

Kyoto Pharmaceutical Industry Co., Ltd. is a comprehensive manufacturer related to clinical testing. We engage in the development, manufacturing, sales, and import/export of bio-related products such as clinical testing reagents, culture media and reagents for bacterial testing, medical equipment, and cell culture-related products, delivering advanced and reliable products to customers involved in clinical testing and life sciences. In our manufacturing, we are passionate about quality, sincerely respond to customer needs, prioritize kindness to people, and consider the future in relation to the environment, aiming to be a company that contributes to society through our business activities. 【Business Description】 ■ Development, manufacturing, sales, and import/export of clinical testing reagents (in vitro diagnostic pharmaceuticals), culture media and reagents for bacterial testing, medical equipment, and bio-related products such as cell culture-related products. *For more details, please refer to the PDF document or feel free to contact us.

• Other bio-related products and services
• Other bio-related products

It has been demonstrated that using it in combination with "CP-5E" for the cryopreservation of human iPS/ES cells results in a high survival rate after thawing. Due to its broad substrate specificity and ability to degrade various proteins, it can also be applied to other uses. 【Features】 ■ Usable for both on-feeder and feeder-free cultures ■ Capable of adjusting the dispersion state from single cells to uniform cell aggregates ■ Ready-to-Use ■ Free from animal-derived materials *Please contact us if you would like a sample. *For more details, please contact us or refer to the catalog.

• Other research reagents
• Other bio-related products and services

By combining it with the cell dispersion solution "Pronase/EDTA for Stem," a high survival rate can be achieved. The composition is clear, so you can use it with confidence. 【Features】 ■ Enables cryopreservation of various cells, including human iPS/ES cells ■ Usable for human iPS cells cultured without feeders ■ Allows cryopreservation with a small number of cells ■ Reduces differentiation risk by keeping DMSO concentration at 5% ■ Free from animal-derived materials 【Examples of cells that can be preserved with CP-5E】 ■ Human iPS cell lines (253G1, 201B7*) ■ Human ES cell lines (KhES-1*, H1) ■ Mouse adipocyte precursor cell line (3T3-L1*) ■ Human pancreatic cancer cell line (PANC-1) ■ Primate kidney-derived cell line (COS-7) ■ Human fetal lung-derived fibroblast cell line (WI-38) ■ Human fetal lung-derived fibroblast cell line (MRC-5) ■ Human fetal kidney-derived cells (HEK-293) ■ Mouse feeder cells (SNL76/7) ■ Human myeloid leukemia cell line (HL-60*) ■ Human NK cell line (NK-92MI) (* Differentiation ability confirmed after freezing and thawing) *For more details, please contact us or refer to the catalog.

• Other research reagents
• Other bio-related products and services
• reagent

At Kyokuto Pharmaceutical Industrial Co., Ltd., we manufacture media with specified compositions for biopharmaceutical production, regenerative medicine, vaccine production, and more. 【Features】 ■ Over 40 years of trust and experience ■ Excellent grinding technology ■ Traceability of raw materials ■ Thorough manufacturing and quality control *For more details, please refer to the catalog or feel free to contact us.

• Culture medium
• OEM
• Other bio products