sainome List of Products and Services

In-gel digestion is a fundamental and important operation in proteome analysis, yet it requires a high level of skill. As a result, veterans in the laboratory engage in this operation, leaving them with little time for their original research. By using SAINOME for this operation, it becomes possible to dramatically reduce keratin contamination and improve reproducibility while maintaining the number of identifications, even for those who are not skilled. This is achievable because the cutting and dispensing of the gel can be done in a single action, significantly reducing contamination. Additionally, simultaneous labeling prevents mix-ups of the cut gels, further enhancing reproducibility. The reduced number of operational steps and the ability to use pipettors around the 384-well plate contribute to a reduction in operation time as well. Main implementation results → Yokohama City University, National Cancer Center, and others.

• Other bio materials

- Zymography (DEG Method) When using zymography to detect new enzymes, conventional zymography has low sensitivity, making it impossible to detect trace amounts of enzymes (activity). By using SAINOME and fluorescent reagents in this system, the sensitivity increases by about 1,000 times, allowing for the detection of trace proteins below 0.1 ng. While fluorescent dyes have high sensitivity, their low molecular weight causes the increase in fluorescence due to turnover to be overwhelmed by diffusion into the gel, making detection impossible. By cutting and dividing the electrophoresis gel with SAINOME before the reaction, it is possible to prevent the diffusion of fluorescence due to turnover, allowing for accumulation and enabling the detection of trace enzyme activity. This method is called the DEG method, and many new enzymes have been discovered using it. By utilizing the DEG method, it becomes possible to comprehensively evaluate enzyme activity within samples. Comprehensive comparative evaluations between patients and healthy individuals (or between cancerous and normal tissues) can be conducted, and it is used in drug discovery target exploration. Main implementation results → Graduate School of Pharmaceutical Sciences, The University of Tokyo

• Other bio materials
• Protein Extraction Kit
• Other analytical and testing equipment

"Sainome" is a useful analysis tool for proteome analysis and DEG assays. After electrophoresis, the gel can be dispensed into a 384-well microplate and used directly for reactions or measurements with a microplate reader. In addition to a black type suitable for fluorescence measurements, we also offer a clear type suitable for observing stained gels. Please feel free to contact us if you have any requests. 【Features】 ■ Achieves detection of trace proteins below 0.1 ng due to high sensitivity ■ Significantly reduces the hassle of cutting gels ■ Analyzes proteins in the target wells *For more details, please download the PDF or feel free to contact us.

• Other analysis and sequence
• Other Analysis