オレンジサイエンス List of Products and Services

We also provide technical support for all products, and we are a trading company closely associated with the manufacturers. The main products we handle are as follows: - Muscle cell electrical stimulation culture device 'IonOptix/ C-Pace' - Cell stretching and pressure stimulation culture device 'STREX/ ST/SP series' - Time-lapse fluorescence microscope within an incubator 'etaluma/ Lumascope' - Tissue section slicer 'Precisionary/ Compresstome' - Small animal lung function analysis device 'emka/SCIREQ/ flexiVent' - Small animal respiratory measurement device with plethysmography 'emka/SCIREQ/ vivoFlow' - Isolated cardiac myocyte sarcomere Ca measurement system 'IonOptix/ Myocyte System' - iPS cardiac myocyte motion vector 'IonOptix/ CytoMotion' - Microscale compression testing machine 'CellScale/ MicroTester' - Liquid tensile compression testing machine 'CellScale/ UniVert' and more.

• Other Culture
• Other animal and plant experimental equipment and instruments

By embedding the tissue in agarose and slicing vertically while minimizing Z-directional wobble, it is possible to slice without damaging the tissue and without compromising its inherent functions. 【Features】 ■ Embedding samples in agarose ■ Slicing in the vertical direction ■ Minimizing Z-directional wobble with Auto Zero Z technology ■ Controlling the extrusion speed of the sample and blade vibrations ■ As a result, it allows for live analysis by minimizing damage to the sample during slicing.

• Other cells

The "MicroTester" is capable of performing compression tests on spheroids, gels, microbeads, and other materials sized approximately 50 to 500 µm. The "UniVert" can conduct tensile and compression tests (with an optional three-point bending test) using a single unit. By adding an optional liquid measurement bath, it allows for measurements in liquid both vertically and horizontally. The "BioTester" is a dual-axis tensile testing machine. It secures samples with a tool resembling a rake and can pull in two directions while measuring the force. 【Achievements】 ■ Used in a paper by the Department of Cardiovascular Surgery at Osaka University for measuring the contraction force of iPS cardiac tissue. ■ Used in a paper by the Department of Ophthalmology at Sapporo Medical University for measuring the hardness of spheroids. ■ Used for measuring the hardness of microbeads at a major bioreactor manufacturer. ■ Used for measuring the hardness of gels at a major pharmaceutical manufacturer. 【Features】 ■ The dedicated software allows for setting the time for loading, holding, and unloading. ■ The standard camera enables synchronization of data and images. ■ High-resolution cameras allow for strain mapping (optional for UniVert and BioTester). ■ Measurements can be conducted in liquid (optional for UniVert). *For more details, please refer to the PDF document or contact us.

• Other physical property measurements and component analysis
• Other analytical and testing equipment
• Other Physics and Chemistry Equipment

We would like to introduce the "Bath system" that we handle. The measurement of tissue contractility through drug administration is widely used in preclinical research, and studying the inherent ability of tissues to contract or relax in an independent tissue bath, free from external influences, can provide supplementary data to in vivo measurements. This system is suitable for the study of tissue samples such as smooth muscle (aortic ring, ileum, uterine tissue, trachea, mesenteric artery, colon), cardiac muscle (atria, ventricles, papillary muscles), and skeletal muscle (diaphragm), as well as blood vessels. 【Features】 <emkaBATH4: Tissue Bath 4ch System (Auto Magnus)> ■ Tension adjustment (via vernier positioner) ■ Pre-heating of buffer ■ Temperature control of the bath ■ Buffer filling and drainage *For more details, please refer to the PDF document or feel free to contact us.

• Other analytical and testing equipment
• Other measurement and measuring equipment

We would like to introduce "easyTEL," which we handle. The IOX data acquisition and analysis system can receive signals from up to 16 implants and allows monitoring from multiple remote PCs. The Easy Matrix Interface can connect up to 16 easyTEL receivers, equipped with 8 bridge amplifiers, enabling simultaneous acquisition of signals such as breathing. 【Features】 ■ Receives signals from up to 16 implants ■ Can be monitored from multiple remote PCs ■ Transmission range: 20cm (depending on radio environment) ■ Capable of synchronizing video with signals ■ Target animals: small animals such as rats and mice *For more details, please refer to the PDF materials or feel free to contact us.

• Other analytical and testing equipment
• Other measurement and measuring equipment

『inExpose』 is an inhalation exposure system designed to expose small animals (mice, rats, guinea pigs) to aerosols, mixed gases, tobacco smoke, and more. It combines necessary equipment such as exposure towers for the nasal area of each animal, whole-body exposure chambers, nebulizer units, mixed gas adapters, and tobacco smoke robots, all controlled by a computer. The exposure conditions are fully managed by the computer, and the controller can accommodate up to four pumps. 【Features】 ■ Capable of nasal and whole-body exposure ■ All exposure conditions are managed by the computer ■ The controller can accommodate up to four pumps *For more details, please refer to the PDF materials or feel free to contact us.

• Other gas generators

We would like to introduce our non-restrictive respiratory function analysis device for mice, 'VivoFlow'. By coloring the chamber yellow, it has been confirmed that more stable respiratory function measurements can be obtained compared to a transparent chamber, especially in mice. By adding a gas mixing device that employs a mass flow controller, it is possible to measure changes in respiratory function during mixed gas exposure. 【Specifications】 ■ Target animal: Mouse ■ Number of animals measured simultaneously: 1 to 8 ■ GMS: Gas Mixing System ■ GMS2: Two-gas mixing system (two types from N2, O2, CO2) ■ GMS3: Three-gas mixing system (N2, O2, CO2) *For more details, please refer to the PDF document or feel free to contact us.

• Other analytical and testing equipment
• Other measurement and measuring equipment

The "flexiVent" is a respiratory and pulmonary function analysis device that achieves the highest level of sensitivity and reproducibility by precisely controlling experimental conditions. Beyond the general measurements of airway resistance and dynamic lung compliance, it provides important detailed data on the actual mechanical properties, including information from both central and peripheral airways. There are six types of modules available, tailored to the size of animals such as mice, rats, guinea pigs, ferrets, and piglets. 【Features】 ■ Equipped with a mechanically ventilated system fully controlled by a computer ■ Capable of precisely analyzing changes in lung conditions from the airways of various animals ■ Utilizes the Forced Oscillation Technique (FOT) for animal experiments ■ Enables analysis of central airways and peripheral lungs *For more details, please refer to the PDF document or feel free to contact us.

• Other analytical and testing equipment
• Other measurement and measuring equipment

Binaree's Tissue Clearing Kit is a solvent that can make tissues transparent. Since it does not use organic solvents, it can also be used for genetic analysis. For mouse brains, it can be processed in a few days with standard product solvents, or in 6 hours when used in combination with the Rapid system! In addition to zebrafish, mouse, and rat brains, various other tissues can also be made transparent.

• Other research reagents

Organomation's "Nitrogen Blowdown Evaporator" is a nitrogen evaporator that can process a large number of samples at once. There are mainly three models: the "N-EVAP" for multiple different sizes of test tubes, the "MICROVAP" for microplates, and the "MULTIVAP" for high-capacity and high-speed processing. The "MULTIVAP" is a model that can process large volumes quickly at once. Depending on the model, it can handle between 9 to 100 samples at a time. For detailed specifications, please refer to the catalog or our website. 【Features】 ■ Available capacities: 9, 30, 48, 64, 80, and 100 samples ■ Evaporation of solvents using nitrogen blowdown and a water bath or drive block heater *For more details, please download the PDF or feel free to contact us.

• Evaporator

Organomation's "Nitrogen Blowdown Evaporator" is a nitrogen evaporator that can process many samples at once. There are mainly three models: the "N-EVAP" for multiple test tubes of different sizes, the "MICROVAP" for microplates, and the "MULTIVAP" for high-capacity and high-speed processing. The "MICROVAP" can process 96-well multi-plates at once, and there is also a model that can process three multi-plates simultaneously. For detailed specifications, please refer to the catalog or our website. 【Features】 ■ Available configurations: 15 or 24 tubes, and 1 or 3 pieces of 96-well multi-plates ■ Evaporation of solvents using nitrogen blowdown and a drive lock heater *For more details, please download the PDF or feel free to contact us.

• Evaporator

The "Nitrogen Blowdown Evaporator" is a nitrogen evaporator that can process multiple test tubes of different sizes at once. There are mainly three models: the "N-EVAP" for multiple test tubes of different sizes, the "MICROVAP" for microplates, and the "MULTIVAP" for high-capacity and high-speed processing. The "N-EVAP" can accommodate different sizes of test tubes, with options for 6, 12, 20, 24, 34, or 45 tubes to be processed at once. The size of the test tubes that can be set varies by model, so please refer to the catalog or website for detailed specifications. 【Features】 ■ Available configurations for 6, 12, 20, 24, 34, or 45 tubes ■ Evaporation of solvents using nitrogen blowdown and a constant temperature bath ■ The constant temperature bath is made of stainless steel ■ The 34 and 45 Position models allow for digital control of bath temperature and processing time ■ The 45 Position model features quick drain capability *For more details, please download the PDF or feel free to contact us.

• Evaporator

CellScale's BioTester is a device for biaxial tensile testing. Samples are secured on all sides with a BioFlake resembling a rake, and pulled by actuators on each axis, while a load cell measures the strength. The platform for securing the samples is equipped with a temperature-controlled bath, allowing for measurements in liquid, and a camera on top captures the displacement of the samples. The BioTester5000 is a full system, while the BioTester3000 can be customized with additional features to fit budgetary needs. Features: ■ Sample holding fixture (BioFlake) ■ Real-time data acquisition ■ Software capable of controlling displacement and force ■ Temperature-controlled chamber ■ High-performance camera for image analysis ■ Software capable of quantifying and visualizing displacement ■ Easily disassembled for cleaning

• Other measurement and measuring equipment

Flexcell's Flex Jr. allows for the cultivation of cells seeded on a dedicated soft bottom plate (BioFlex) by stretching them under vacuum pressure from a vacuum pump, providing mechanical stretch stimulation to the cells. With dedicated computer software, stimulation protocols and waveforms can be easily set, enabling the circular culture surface to be stretched 360 degrees, thus providing stimulation that is very close to in vivo conditions. Features: - Compatible with dedicated 6-well and 24-well plates - Stretchable up to 21.8% (up to 33% without using Loading Posts) - The higher model FX-6000T can use 4 plates simultaneously (up to 8 plates with additional baseplate) - The FX-6000T also allows for uniaxial stretching using a uniaxial stretch plate (UniFlex)

• Culture Equipment

The gas pressurization stimulation culture device from STREX is a system consisting of a dedicated pressure chamber and a controller. The chamber is pressurized at the pressure set by the controller. Since the gas in both the incubator and the chamber is replaced, pressurized stimulation culture can be performed under incubator conditions. It is also possible to connect two chambers to one controller, and by using a separately sold stainless steel chamber, stimulation at higher pressures is achievable. 【Features】 ■ Can be installed in a CO2 incubator for long-term pressurized culture. ■ Pressure range of 0 to 350 mmHg, allowing for the setting of two pressure values for pressure adjustment. ■ Various stimulation protocols can be easily set using the controller. *For more details, please download the PDF or feel free to contact us. Related link - https://www.orangescience.co.jp/strex-%E3%82%AC%E3%82%B9%E5%8A%A0%E5%9C%A7%E5%88%BA%E6%BF%80%E5%9F%B9%E9%A4%8A%E8%A3%85%E7%BD%AE

• Other Culture

The IonOptix CytoMotion system attaches a dedicated camera to the C-Mount of your microscope, captures the measurement range with original software, detects pixel displacement, and measures cell movement. By adding it to the MyoCyte system, Ca measurement also becomes possible.

• Cell Analysis Software

The STREX device is designed to stretch a silicon chamber and culture cells that have been seeded on it while providing stretching stimuli. In addition to the manual stretching ST-100 model, there are also the ST-1400 and ST-1440 models that can automatically provide 64 patterns of stretching stimuli, as well as single-axis and dual-axis stretching models for inverted microscope observation.

• Other cell research

CellScale's MCTR is a device that allows for cell culture under hydrostatic pressure. Cells are placed in a 9-well pressure chamber filled with culture medium, covered with a PTFE membrane, and hydrostatic pressure is applied to the cells from above the membrane using pressure from an air compressor. 【Features】 ■ Pressure can be applied from 50 to 700 KPa (0.05 to 0.7 MPa) ■ Dedicated software allows for easy setting of load, hold, and unload times and distances ■ The pressure chamber, O-ring, and membrane are autoclavable and reusable *For more details, please download the PDF or feel free to contact us. Related link - https://www.orangescience.co.jp/cellscale-mechanoculture-tr

• Other cell research

With over 20 years of numerous achievements both domestically and internationally, the MyoCyte system enables the measurement of cell contraction and sarcomeres through image analysis, as well as the fluorescent measurement of cells using a two-wavelength light source with a ratiometric method. In recent years, a fully automated model called the MultiCell system, which incorporates this functionality along with a high-performance camera, has also been released.

• Other Analysis

We handle the "Mechanoculture Series" stretching and compression stimulation culture devices from Cellscale. All products can be connected to your computer to set up stretching protocols. After setting, you can disconnect the computer and place the device inside an incubator for use. 【Product Lineup】 ■ Mechanoculture B1 (MCB1) - 360-degree stretching stimulation culture device ■ Mechanoculture T6 (MCT6) - Sheet material stretching stimulation culture device ■ Mechanoculture FX (MCFX) - Uniaxial stretching stimulation culture device ■ Mechanoculture TR (MCTR) - Fluid compression stimulation culture device ■ Mechanoculture TX (MCTX) - Mechanical compression stimulation culture + mechanical measurement recording device ■ Mechanoculture J1 (MCJ1) - Stretching stimulation culture + mechanical measurement recording device *For more details, please download the PDF or feel free to contact us.

• Culture Equipment

The "Lumascope 820/850" from Etaluma is a compact time-lapse fluorescence microscope designed for use in limited spaces such as incubators, safety cabinets, and environmental control workstations. With an LED light source, Semrock filters, advanced optical design, and a CMOS sensor, it achieves nearly diffraction-limited resolution. Additionally, by simply connecting to a dedicated computer via USB, it allows for easy saving of still images, time-lapse sequences, and videos, enabling low-cost, high-quality imaging in three-color fluorescence (blue, green, red). 【Features】 - Reduces costs and simplifies operation and maintenance through advanced LED optical design. - Achieves quick setup and ease of use with power supply and control via USB connection to a PC. - Provides excellent resolution even under general lighting conditions with an advanced optical system. - Enhances observation of unstained samples with a phase contrast unit. - Offers high image quality through compatibility with objectives from Evident, among other features. *For more details, please download the PDF or feel free to contact us.

• Other microscopes and optical inspection equipment

The "Microtester" from CellScale is a system capable of measuring the tensile and compressive strength of various materials such as tissue samples, cell spheroids, hydrogels, and scaffolds, as well as performing image analysis of displacements using a camera positioned in front of the chamber. By detecting minute changes with a force sensor and micro-wires, it can measure smaller forces and displacements than conventional measuring devices. 【Features】 ■ Equipped with a high-quality optical system that allows for user feedback ■ Force resolution down to a minimum of 10 nN, spatial resolution up to 0.1 μm ■ Control software supports protocols for both force and displacement control *For more details, please download the PDF or feel free to contact us.

• Other measurement and measuring equipment

The "Univart" from Selscale is a tension and compression strength measurement system suitable for a wide range of mechanical tests in limited spaces. Due to its compact and affordable design, tests can be conducted anywhere, whenever needed. Additionally, both the software and modular components are easy to use, so no special training is required. 【Features】 - By changing the load cell, tensile and compression tests of up to 200N can be performed. - Various attachments for fixing samples and years of expertise allow for compatibility with different samples and test modes, among other things. *For more details, please download the PDF or feel free to contact us.

• Other measurement and measuring equipment

The "C-Pace System (C-Pace EM)" is a system that allows for cell culture while providing electrical stimulation according to a protocol pre-set in the main unit (C-Pace EM), using carbon electrodes (C-Dish) compatible with standard culture dishes. The main unit has one built-in power board, but it is possible to expand it to a maximum of eight power boards, allowing for different protocols to be applied for electrical stimulation for each power board (per dish). 【Features】 ■ Suppression of electrotoxic effects through bipolar pulses ■ Prevention of electrolyte decomposition with carbon electrodes ■ Maximum current value: 240mA ■ Input and output of external trigger signals possible ■ Connection to C-Dish via flat cable, etc. *For more details, please download the PDF or feel free to contact us.

• Culture Equipment