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"BIO ONE" is a product that features cooling capabilities down to 0°C and allows for precise dispensing using syringes. It enables the dispensing and shaping of temperature-sensitive materials such as Matrigel into 384-well plates and similar formats. It allows for a smooth transition to three-dimensional cell culture. Designed for the dispensing and printing of highly temperature-sensitive materials like Matrigel and collagen, it enables the creation of 3D culture models that replicate the in vivo environment. Additionally, it supports printing with soft materials. 【Features】 ■ High-precision dispensing ■ Head cooling function ■ Stage heating function ■ Compatibility with various materials ■ User-friendly proprietary software *For more details, please refer to the PDF document or feel free to contact us.

• Printing Machinery

The "BIONOVA X" is a type of bio 3D printer that cures hydrogels using DLP light crosslinking. It has a high resolution of about 10μm and also supports grayscale printing (changing the illumination according to the pixel intensity in the model image). Developed in the lab of Dr. Shaochen Chen at UCSD, this device accommodates a variety of applications and can be applied to the development of axonal guides for spinal injury treatment. 【Features】 ■ Can print directly onto well plates with up to 24 wells ■ Provides an ideal environment for high-throughput applications ■ Capable of easily modeling tiny structures and complex shapes such as vascular systems ■ Patented continuous printing technology ■ Achieves overwhelming speed without sacrificing print fidelity *For more details, please refer to the related links or feel free to contact us.

• 3D Printer

This document introduces examples of application analysis using the advanced solution "CELLCYTE X" to maximize insights from cell culture experiments. The section "Simple Workflow of CELLCYTE X" covers STEP 1 to STEP 5, providing clear explanations with photos and diagrams. We invite you to read it. [Contents] ■ Cell toxicity assay using C.LIVE Tox reagents ■ Simple workflow of CELLCYTE X ■ Real-time simultaneous analysis of Live/Dead Cell Assay with two fluorescence channels *For more details, please refer to the PDF document or feel free to contact us.

• Other analyses
• Contract Analysis
• Contract Analysis

Cell culture, which is essential in fields such as regenerative medicine, oncology using stem cells, drug discovery, and toxicity evaluation, is still predominantly conducted in two-dimensional cultures. However, some may wonder if conducting experiments in three dimensions, similar to the cells themselves and the surrounding environment in vivo, would allow for a more faithful study of cell behavior. In this document, we introduce the importance of three-dimensional culture and our company's 3D bioprinting technology, as discussed with our application specialists. We encourage you to read it. [Contents (partial)] - Highly flexible 3D bioprinting - Usable even by beginners, allowing for plenty of trial and error - Extrusion printer BIO X - Light-curing bioprinting LUMEN X+ *For more details, please refer to the PDF document or feel free to contact us.

• 3D Printer

"INKREDIBLE" is an entry-level bio 3D printer that can continuously print multiple types of cells with a dual print head. It efficiently UV crosslinks using a 365nm or 405nm LED light source. It features a space-saving design that can be installed in a clean bench. With high-precision XYZ control, it enables accurate cell layering and supports both standalone control and PC control. [Features] - Continuous printing of multiple types of cells with a dual print head - Efficient UV crosslinking with a 365nm or 405nm LED light source - Space-saving design suitable for installation in a clean bench - High-precision XYZ control for accurate cell layering - Compatible with both standalone and PC control *For more details, please refer to the PDF materials or feel free to contact us.

• 3D Printer
• Other machine tools
• Other physicochemical equipment

We would like to introduce the application examples of the fluorescent reagent 'C.LIVE' for cell toxicity assays, which enables real-time direct detection of cytotoxicity. It enters dead cells through damaged plasma membranes and emits fluorescence after binding to DNA. There is no impact on the growth and morphology of live cells upon addition. Perform cell toxicity assays in a simple three-step process: seed the cells, add compounds containing this reagent, and dynamically monitor the cell status using the CELLCYTE X imaging system. It is suitable for long-term live cell experiments. 【Application Examples】 ■ Measurement of the toxic effects of various cytotoxic drugs or physical stress on cells - Addressing the need for real-time cytotoxicity analysis in incubators, primarily in the pharmaceutical industry. *For more details, please refer to the PDF document or feel free to contact us.

• Other consumables

In a cytotoxicity assay, the toxic effects of various cytotoxic drugs or physical stress on cells are measured. 'C.LIVE TOX GREEN/RED' is a highly sensitive DNA-binding dye that is impermeable to live cells and non-fluorescent outside the cells. It enters dead cells through damaged plasma membranes, binds to DNA, and emits fluorescence. The addition of the reagent does not affect the growth and morphology of live cells. 【Features】 ■ Direct detection of dead cells by the reagent ■ Suitable for long-term live cell experiments ■ Simple mix-and-read workflow ■ Dynamic monitoring of cytotoxicity by measuring fluorescent substances over time *For more details, please refer to the PDF document or feel free to contact us.

• Other consumables

"PureCol" is a collagen (extracellular matrix) for cell culture that can be used for coating cell culture containers and preparing 3D gels. PureCol Type I Collagen "#5005-100ML" is suitable for coating "CytoSoft plates." We offer both solution type (concentration: 3mg/ml, 100ml) and freeze-dried type (15mg). For more details, please feel free to contact us. 【Features】 ■ Purity >99.9% ■ Cited in over 2000 research papers ■ Excellent lot-to-lot reproducibility *For more information, please refer to the PDF document or feel free to contact us.

• Coating Agent

The "CytoSoft Imaging Plate" allows for the acquisition of high-resolution microscopic images using high numerical aperture lenses. It is compatible with #1.5 glass substrates and 24 and 96 well plates. It features a thinner silicon layer. For more details, please feel free to contact us. 【Features】 ■ #1.5 glass substrate ■ Thinner silicon layer ■ Compatible with 24 and 96 well plates ■ Capable of acquiring high-resolution images with high numerical aperture lenses *For more information, please refer to the PDF document or feel free to contact us.

• Other laboratory equipment and containers

We would like to introduce the "CytoSoft Plates and Flasks" that we handle. We have selected and introduced a 6-well plate with an appropriate softness, allowing for a precise analysis of cell behavior and identification of the optimal rigidity level. For long-term culture experiments, you can use the "CytoSoft Plate," and for large-scale cultures, the "T25/T75 Flask Version" is available. Please feel free to contact us for more details. 【Features】 ■ Selected and introduced a 6-well plate with an appropriate softness ■ Conducted a precise analysis of cell behavior and identified the optimal rigidity level ■ Application of "CytoSoft Plates" for long-term culture experiments ■ Use of "T25/T75 Flask Version" for large-scale cultures * For more details, please refer to the PDF materials or feel free to contact us.

• Other laboratory equipment and containers

The "CytoSoft Discovery Kit" consists of a set of 7 plates in a 6-well format that covers physiological stiffness, with stiffness levels of 0.2, 0.5, 2, 8, 16, 32, and 64 kPa. When dermal fibroblasts were cultured on CytoSoft plates at 0.2, 8, and 64 kPa, significant formation of F-actin stress fibers was observed in the 0.2 kPa and 64 kPa environments. On the other hand, fibroblasts at 8 kPa showed increased adhesion to the substrate, resulting in a reduction of stress fibers. 【Features】 ■ Tested under all stiffness conditions ■ Observation of differences in cell behavior *For more details, please refer to the PDF document or feel free to contact us.

• Other laboratory equipment and containers

The "CELLCYTE X" is a live cell imaging system that efficiently and high-throughput analyzes live cells. To easily obtain highly reproducible data, we have prepared an efficient workflow. It can operate within a standard CO2 incubator. The open platform design enables imaging with various sample mount shapes. 【Features】 ■ High cell viability ■ Increased productivity ■ High-quality quantitative data ■ Real-time analysis of cells in optimal conditions ■ Intuitive operation achieved with two software programs *For more details, please refer to the PDF document or feel free to contact us.

• Software (middle, driver, security, etc.)
• Other analyses

The "CELLCYTE X" is a high-throughput live cell imaging system that pursues efficiency, affordability, and ease of use. Cell biologists often conduct experiments using endpoint assays, drawing conclusions about cellular behavior at a single point in time. By using this product installed in an incubator, images obtained from multiple time points can be stitched together for playback, allowing for more accurate tracking of a series of biological events and a comprehensive understanding of cell dynamics. 【Features】 ■ Easy maintenance and control of the cellular environment ■ Reduced incidence of cell abnormalities due to minimal disturbance during experiments ■ Data collected and processed in real-time throughout the experiment ■ Simultaneous processing of six well plates, achieving maximum throughput ■ Equipped with a 5-megapixel camera (compatible with 4x and 10x objectives) ■ Compatible with T25/75 flasks and micro well plates *For more details, please refer to the materials. Feel free to contact us with any inquiries.

• Software (middle, driver, security, etc.)

Introducing application examples of the liquid handling system "I.DOT Series." The "I.DOT Series" is a non-contact liquid handling system that automates the dispensing of various liquids, from reaction reagents to cell suspensions, in micro-volumes. One application example is the "miniaturization and automation of cell-based assays." It completes cell seeding, culture, and analysis on a droplet microarray (DMA) slide. 【Features】 ■ Non-contact ■ Compatible with various solutions ■ Reagent and sample savings ■ Flexible ■ High-speed processing ■ Droplet detection function *For more details, please refer to the PDF document or feel free to contact us.

• Other machine elements

We are introducing application examples utilizing the BIO X series 3D bioprinter. This product allows for flexible use tailored to research purposes across a variety of applications. There are numerous research cases, including skin models, various organ models (such as heart, liver, kidney, etc.), and cancer disease models. [Contents] - Bioprinting of primary human mesenchymal stem cells and vascular endothelial cells - Multilayer tissue models using multiple cells and materials - Encapsulation of cells and molecules - High-precision automatic dispensing of Matrigel *For more details, please refer to the PDF materials or feel free to contact us.

• 3D Printer

In Dr. Carmine Gentile's laboratory in Sydney, research is being conducted to develop bio-printed heart tissue using patient-specific cells to minimize the risk of transplant rejection. In a 2020 study, mouse cells were bio-printed using alginate-gelatin hydrogel with CELLINK's "BIO X." The bio-printed heart cells were cultured for 7 to 14 days until they developed into pulsating patches of heart tissue. During open-heart surgery on mice from which the cells were derived, the vascularized heart patches were transplanted into the damaged areas of the heart. The transplantation aimed to test the safety of the bio-printed patches and confirm their positive effects on heart function, both of which were validated. [Summary] - Facility: Dr. Carmine Gentile's laboratory in Sydney - Challenge: Development of bio-printed heart tissue - Results: - Safety of the bio-printed patches - Positive effects on heart function *For more details, please refer to the related links or feel free to contact us.*

• 3D Printer

Current artificial skin graft products promote wound healing, but they ultimately detach because they do not integrate with the patient's skin tissue. The lack of a functional vascular system in artificial grafts poses a significant barrier. We have developed a bioink composed of human endothelial cells, human pericytes, and animal collagen, allowing us to print these structures using the "CELLINK BIO X" and a temperature-controlled print head. This combination of critical elements enables cells to initiate communication and begin forming vascular structures with characteristics similar to biological blood vessels within weeks. Once the vessels are formed, they can exchange nutrients and waste, sustaining the survival of the graft. [Overview] ■ Institution: Rensselaer Polytechnic Institute ■ Challenge: The lack of a functional vascular system in artificial grafts is a significant barrier ■ Solution: Using CELLINK BIO X and a temperature-controlled print head ■ Results - Begin forming vascular structures with characteristics similar to biological blood vessels within weeks - Once vessels are formed, they can exchange nutrients and waste, maintaining the survival of the graft *For more details, please refer to the related links or feel free to contact us.

• 3D Printer

CELLINK Corporation introduces how researchers, our customers, are conducting their research using the products and solutions we offer. Using the "CELLINK BIO X," researchers at RPI have made significant progress towards the development of fully transplantable bioprinted skin. Additionally, researchers at RIT are developing bioinks to promote organ regeneration using the "CELLINK BIO X." For more details, please refer to the related links. [Overview] ■ Printing of vascularized skin - Researcher: RPI researchers - Product used: CELLINK BIO X ■ Development of bioinks to promote organ regeneration - Researcher: Professor Iris Rivero from RIT - Product used: CELLINK BIO X *For more information, please refer to the related links or feel free to contact us.

• 3D Printer

We would like to introduce our bio 3D printer, the "BIO X Series." We offer the "BIO X" and "BIO X6," which allow for flexible use according to research purposes. As an application example, in "cell and molecule encapsulation," cells, drugs, proteins, spheroids, and more can be embedded within hydrogel beads. Additionally, it is possible to print multiple cells and materials using up to six print heads. 【Flexible Use According to Research Purposes】 ■ Utilize multiple print heads ■ Continuously print models ■ Create flexible 3D models ■ Dispense materials like Matrigel *For more details, please refer to the PDF document or feel free to contact us.

• others

"UP.SIGHT" streamlines the Cell Line Development (CLD) workflow by automating labor-intensive and time-consuming steps. Our patented technology enables high-efficiency, rapid, and gentle single-cell dispensing while simultaneously capturing images of the entire well (with no need for image stitching and providing ultra-fast, high-quality imaging). In our all-in-one solution, two optical devices (cameras) capture three-dimensional images of the nozzle and the entire well, respectively. This allows for a dual assurance of clonality, increasing the establishment of clonality to over 99.99%. 【Features】 ■ Improved cell line development workflow ■ Two-step verification of clonality ■ Reduced risk of cross-contamination ■ High yield, excellent insights, and fast processing ■ Cell focusing - minimizing cell loss *For more details, please refer to the PDF document or feel free to contact us.

• Other cell research

The "I.DOT Series" is a non-contact liquid handling system that achieves robust, simple, and highly reliable dispensing using a non-contact method based on air pressure. It accommodates complex liquid handling tasks with a new approach. Please feel free to contact us if you have any inquiries. 【Features】 ■ Non-contact ■ Compatible with various solutions ■ Flexible ■ High speed *For more details, please download the PDF or contact us.

• others

The "CELLINK(R) based bioink" is a universal bioink optimized for 3D bioprinting of human tissues, which can be used with various commercially available or in-house 3D bioprinting systems. As a polysaccharide hydrogel (non-animal derived), CELLINK(R) is suitable for 3D cell culture due to its biocompatibility and ease of bioprinting. It is also used for a wide range of cell types, and its biological functionality has been widely evaluated in tissue engineering applications such as bone, skin, and tumor engineering. 【Product Lineup】 ■CELLINK ■CELLINK BONE ■CELLINK FIBRIN ■CELLINK RGD ■CELLINK SKIN, etc. *For more details, please download the PDF or feel free to contact us.

• others

The "BIO X" is a desktop bio 3D printer equipped with an intelligent print head (iPH) that can accommodate a wide range of materials. It features two high-output fans at the top of the device, which pressurize the cavity with clean air filtered through HEPA filters and other means. Additionally, it is equipped with UV-C lamps that maintain a clean printing environment through an automatic cycle. 【Features】 ■ Compatible with temperature-sensitive materials such as collagen and gelatin ■ A wide lineup of high-performance print heads ■ Easy replacement of print heads ■ Suitable for a wide range of applications *For more details, please refer to the materials. Feel free to contact us with any inquiries.

• others