We will introduce some detailed points of caution regarding experimental methods such as swimming motion and antibody detection.
The "Western blotting method" is a technique used to specifically detect target proteins by transferring protein samples onto a membrane after electrophoresis and using specific antibodies. This document, "Tips for Chemiluminescence Detection: Western Blotting Edition," outlines the use of the chemiluminescence imaging device "AE-6971/2 Light Capture" for detection, as well as detailed precautions regarding experimental methods such as electrophoresis and antibody detection. [Support for resolving the following issues] ■ Regarding band detection status: - The target band is faint and cannot be confirmed. - High background or unevenness prevents confirmation of the target band. ■ Issues with capturing luminescence patterns: - Adjusting exposure with X-ray film is difficult. - X-ray film makes data reading and analysis challenging. For more details, please contact us or download the catalog.
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【Contents】 ○ Electrophoresis ○ Western Blotting ○ Blocking ○ Antigen-Antibody Reaction ○ Addition of Luminescent Substrate and Luminescent Detection ○ Photography of Luminescent Patterns ○ Troubleshooting ○ Tips for Western Blotting - Detection with X-ray film can also be applied to other companies' chemiluminescence imaging devices, so we hope this will be helpful to you. For more details, please contact us or download the catalog.
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【Purpose】 ○ As a guidebook for experimental methods such as electrophoresis and antibody detection using the chemiluminescence imaging device. ● For more details, please contact us.
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ATTO is a company that develops, manufactures, and sells scientific instruments for research, such as proteins and nucleic acids. Its traditional representative products include "Peristaltic Pump," "Mini-Slab Electrophoresis Device," "Pajel," and "Print Graph," which are used in research laboratories in Japan and abroad. In the current era of advanced protein research, ATTO is developing innovative and powerful products such as the new tools for gene expression analysis: "Cell Graph," "AB-2550 Chronos," "AB-2250 Luminescence Sensor MCA," and "CLuc."