This is a kit for fractionating and extracting proteins in a stepwise manner from three fractions: "cytosol," "membrane/organelle," and "nucleus" from animal cells.
By fractionation, it can be used to improve protein identification numbers and analyze the intracellular localization of proteins. The extracted proteins can be used for proteomic analysis using mass spectrometry after trypsin digestion and desalting steps. The kit includes four types of extraction buffers, one type of reagent removal buffer, and desalting columns. Features: - Proteins can be extracted from fractions divided into cytosolic, membrane/organelle, and nuclear fractions. - Surfactants can be easily removed. - No ultracentrifugation treatment is required in the fractionation process.
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basic information
Cat. No. 7510-11400 Unit Size 48 divisions
Price range
P2
Delivery Time
Applications/Examples of results
This is a kit for separating animal cells into three fractions: "cytosol," "membrane/organelle," and "nucleus," and for the stepwise fractionation and extraction of proteins. The fractionation can be used to improve the number of protein identifications and for analyzing the intracellular localization of proteins. The extracted proteins can be used for proteome analysis using mass spectrometry after trypsin digestion and a desalting step. The kit includes four types of extraction buffers, one type of reagent removal buffer, and a desalting column.
Company information
Our company mainly deals with equipment, pretreatment devices, parts, packing materials, analytical columns, solid phases, and peripheral devices related to gas/liquid chromatography. We also offer a wide range of concentration introduction devices for trace components, pretreatment devices, and pretreatment consumables. Please feel free to contact us if you have any requests.