This is a reagent (kit) for the specific visualization of telomeres.
We have developed a polyamide that specifically stains the telomeres of chromosomes, and it has now been commercialized as a kit. The repetitive region of simple base sequences located at the ends of chromosomes is referred to as telomeres, which in vertebrates consists of the repeat sequence (TTAGGG). It has been hypothesized that the length of telomeres is related to cancer and aging (progeria) in individuals, increasing their importance as targets for molecular imaging. A method for detecting telomere DNA on chromosomes using fluorescently labeled peptide nucleic acid (PNA) probes with fluorescence in situ hybridization (FISH) is known; however, PNA is difficult to transfer into cells. A tandem polyamide that specifically binds to the repeat sequence (TTAGGG) of vertebrate telomeres has been reported (2001). By using the fluorescently labeled HPTH59, it is possible to fluorescently stain telomere DNA much more simply and quickly than with PNA FISH or Southern blotting techniques.
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As a result of the research, the genomic background outside the telomere region was low, allowing for more selective staining of telomeres. The development of this kit was advanced with the cooperation of Professor Sugiyama from Kyoto University and Professor Maejima from the National Institute of Genetics as part of the "Innovative Medical Technology Research and Development Grant Program" in fiscal year 2013. A highly reproducible synthetic method was developed to enable the continuous supply of fluorescently labeled polyamides. The compound PIPA, invented by Professor Durbin at the California Institute of Technology, is linked by peptide bonds and quickly transitions to intracellular nuclei. It has minimal non-specific adsorption of the probe, making it superior in specificity and sensitivity compared to conventional DNA probe methods and FISH using PNA probes. Furthermore, PIPA binds to the minor groove of double-stranded DNA, allowing for direct visualization of telomeres without disrupting the telomere structure during the hybridization process. Additionally, the fluorescence intensity reflects the length of the telomeres, enabling semi-quantitative analysis.
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Applications/Examples of results
References Maeshima K, Janssen S, Laemmli UK. EMBO J. 2001, 20:3218-28. Kawamoto Y, Bando T, Kamada F, Li Y, Hashiya K, Maeshima K, Sugiyama H. J. Am. Chem. Soc. 2013, 135:16468-77. Hirata A, Nokihara K, Kawamoto Y, Bando T, Sasaki A, Ide S, Maeshima K, Kasama T, Sugiyama H. J. Am. Chem. Soc. 2014, 136:11546-54.
Line up（2）
| Model number | overview |
|---|---|
| TRed-HPTH59-A | Telomere Staining Kit A |
| TAMRA-HPTH59-B | Telomere Staining Kit B |
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The HiPep Research Institute was established to advance the development of new biochips using peptide arrays, the exploration and clinical application of novel active peptides, the application of peptides to biomaterials, and the development of synthetic vaccines, while providing consultation on research and development in the biomedical field, contract synthesis, analysis, testing, commissioned research, and the sale of equipment, instruments, and reagents related to compound library construction.