DMSO-free! Cryopreservation solution for high survival rate cells
"CrioSkares DMSO-free" is a cryopreservation solution for various types of cells that does not contain proteins or DMSO. It demonstrates a high survival rate in various cultured cells even after thawing, and maintains the pluripotency of stem cells (in an undifferentiated state). Due to the absence of toxicity from DMSO and the influence of proteins, it allows for safe cryopreservation of cells with a high survival rate. [Features] - Does not contain serum or protein components - Approximately 90% survival rate even after thawing - Stem cells can be cryopreserved while maintaining differentiation potential - Confirmed to be free from contamination by bacteria, fungi, and mycoplasma through sterility testing - Can be stored stably for long periods at 4°C *For more details, please refer to the catalog or feel free to contact us.
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basic information
【Overview of Operation Method】 ■ Place the cultured cells in a tube, centrifuge, and remove the supernatant. ■ Add this product and suspend the cells to achieve a concentration of 5×10⁵ to 5×10⁶ cells/ml, then aliquot 1ml into storage tubes. ■ Freeze by placing in a -80℃ deep freezer. ※ For long-term storage, keep in liquid nitrogen. ■ Rapidly thaw the stored cells in a 37℃ incubator while shaking, and centrifuge wash with an appropriate medium (about 10ml). *For more details, please refer to the catalog or feel free to contact us.
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【Purpose】 ■ Freezing preservation solution for cells
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Company information
The establishment of BioVerde Co., Ltd. originated from the selection of the pre-venture research "Development of a Long-term Preservation Solution for Biological Tissues at Room Temperature" at Kyoto University in October 2003 by the Japan Science and Technology Agency. Following the conclusion of this pre-venture period, we founded the venture in March 2006 as a spin-off from Kyoto University to further develop the research and advance it towards commercialization. To date, we have engaged in research and development and manufacturing of new preservation solutions for transplantable cells, biological tissues, and organs using plant-derived polyphenols, as well as the development and manufacturing of food products utilizing green tea, green tea extracts (such as catechins), and similar ingredients.