SNP analysis with a single probe! Specifically quantify the target DNA sequence and apply it to base mutation detection.
"Eprobe/Eprimer" is quenching in a single-stranded state, but emits fluorescence by specifically hybridizing with the complementary strand. When applied to PCR methods or SmartAmp methods, it is a fluorescent probe that can detect the target nucleic acid sequence with extreme sharpness. SNP analysis can be performed with a single probe in one tube. It has a higher Tm compared to unlabelled oligo DNA, enabling the detection of trace somatic mutations using PCR clamping methods. 【Features】 ■ High S/N ratio ■ Fluorescent detection possible through specific hybridization ■ Real-time PCR detection and melting curve analysis possible with a single probe *For more details, please refer to the catalog or feel free to contact us.
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basic information
【Specifications】 ■Delivery Yield: 1.5 nmol (approximately 180 reactions) / 10 nmol (approximately 1200 reactions) ■Compatible Chain Length: 10 to 26 mer ■Base with Dye Modification: Thymine (T) only ■Purification: HPLC purified product ■Shipping Form: Dried product ■Dyes ・Thiazole Yellow (430/450) ・Thiazole Orange (510/530) ・Thiazole Pink (570/590) *For more details, please refer to the catalog or feel free to contact us.
Price range
P2
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Applications/Examples of results
Detection of SNP (single nucleotide polymorphism) and genotyping. ~ A single probe can detect three types: wild, homo, and hetero ~ When the probe design of Eprobe is based on the reference nucleotide sequence, the following three types of waveforms can be obtained due to differences in Tm values: 1. Full match (wild homo) for DNA without mutations 2. One base mismatch (mutant homo) for DNA with SNP mutations 3. Hetero for DNA where only one strand has an SNP mutation For more details, please refer to the catalog or feel free to contact us.
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Company information
Danaform Inc. is a company established in 1998, based on the RIKEN Venture System of the National Research and Development Agency RIKEN, with Yoshihide Hayasaki from RIKEN (who has been the representative director of Danaform Inc. since 2021) at its core. The company is actively engaged in gene analysis and genetic testing, focusing on technologies co-developed by Danaform and RIKEN, including contract analysis using next-generation sequencers such as CAGE-seq and RNA-seq, the sale of fluorescent reagents Eprobe capable of SNP detection, and the sale of various clones, boasting a significant inventory. Moving forward, we aim to refine our proprietary technologies and strive for higher quality research and manufacturing as experts in the field of genetics, with the hope of contributing to people's health.