The imaging spectrometer (ImSpector) allows for the measurement of spectral data for all points in the observation field.
● Line Spectroscopy with the Imaging Spectrometer ImSpector By using an imaging spectrometer, it is possible to rapidly measure spectral data across the entire observation field. ● Use of a high-sensitivity camera for the detector By employing a high-sensitivity camera as the detector, it is possible to minimize light damage to the sample caused by the illumination light during measurement. ● Spectral camera unit can be connected to the microscope camera port Since it can be connected to a C-mount camera port, it can be installed on your existing microscope. ● Additional intermediate tube allows for the installation of an observation camera (optional equipment) By adding an intermediate tube to the microscope, it is possible to install an observation camera in addition to the imaging spectrometer camera. This enables the measurement of spectral data at targeted positions while conducting microscope observations.
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■Basic Specifications (Customizable for wavelength range, resolution, light source, etc.) Spectrometer: Imaging Spectrometer ImSpector V8 Wavelength Range: 380–800nm Wavelength Resolution: 2nm (using 30μm slit) Spatial Resolution: 0.71μm (using 20x objective lens) Detector: High-sensitivity sCMOS camera for scientific use Light Source: Customizable based on the sample being measured Stage Movement Method: 2-axis micro-scanning stage Stage Movement Range: ±75mm for each axis Camera Port for Microscopic Imaging: C-mount camera port Observation Camera (optional): Area color sensor camera
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■Measurement Examples ● Two-dimensional spectral image of cancer cells in rats stained with Hematoxylin and Eosin It is possible to extract and quantitatively evaluate the slight differences in coloration due to the varying staining states of biological tissues, such as cell nuclei and cytoplasm, through analysis of spectral mapping data. ● Two-dimensional fluorescence spectral image of rat kidney cells stained with Alexa Fluor 488 Since fluorescence spectral mapping data can be obtained, it is possible to evaluate not only fluorescence imaging but also the wavelength characteristics of fluorescent dyes that change with the surrounding environment (such as salt concentration, pH, and intracellular and extracellular conditions) simultaneously. ■Basic Data Processing Software Functions (Customizable) - Color image display - Specific wavelength image display - Spectrum display for each point - Observation camera image display - Color calculation function - Color difference display with specific points - Color difference area display with specific points
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