An example of analyzing the expression profiles of mature miRNAs and primary transcripts.
We introduce examples of significant contributions to FANTOM, which has developed and expanded around the field of transcriptome analysis. At FANTOM, we analyzed the expression profiles of mature miRNAs and primary transcripts in numerous primary cultured cells using short RNA-seq analysis and the CAGE method. Furthermore, the CAGE method revealed these promoter sequences. As a result, we demonstrated that miRNA promoters are highly conserved, there is a strong correlation between the expression profiles of mature miRNAs and primary transcripts, and there is a strong correlation in the expression profiles among multiple miRNAs derived from the same primary transcript. *For more details, please refer to the PDF materials or feel free to contact us.*
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【Features of the CAGE Method】 ■ Unique data: Experimental identification of transcription start sites, enabling quantification of promoter activity ■ Wide range of applications: Transcriptome analysis possible in any species ■ Wide dynamic range: Detection of rare transcripts is also possible *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Danaform Inc. is a company established in 1998, based on the RIKEN Venture System of the National Research and Development Agency RIKEN, with Yoshihide Hayasaki from RIKEN (who has been the representative director of Danaform Inc. since 2021) at its core. The company is actively engaged in gene analysis and genetic testing, focusing on technologies co-developed by Danaform and RIKEN, including contract analysis using next-generation sequencers such as CAGE-seq and RNA-seq, the sale of fluorescent reagents Eprobe capable of SNP detection, and the sale of various clones, boasting a significant inventory. Moving forward, we aim to refine our proprietary technologies and strive for higher quality research and manufacturing as experts in the field of genetics, with the hope of contributing to people's health.