Three-dimensional tissue culture such as angiogenesis and organoids.
By using a dedicated culture container (microtranswell) for this device, it is possible to allow the culture medium to permeate into the interior of the cultured tissue or cell layer, similar to blood flow. 【Features】 ■ By conducting cell culture in an environment close to in vivo conditions with this device, the following outcomes are expected: (1) The culture medium can be supplied to deeper parts of the tissue or cell mass, enabling longer culture periods and maintenance of activity. (2) Shear stress from flow allows observation of the mechanical responses of cells, such as angiogenesis, which are difficult to observe in static cultures. (3) Secreted substances from upstream cells can affect downstream cells, enabling sequential reactions. ■ The delivery pressure can be adjusted with a controller. ■ The culture system has a sealed structure, allowing for culture in a horizontal position, so the entire holder can be placed on a microscope stage, enabling observation of the culture area from the side of the culture container (see conceptual diagram). ■ The entire system is compact, allowing it to be installed within your existing incubator, making it easy to try perfusion culture. * When using scaffold materials, it is necessary to select a type that allows for easy permeation of the culture medium. Please contact us for details.
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【Applications】 - Cultivation Results [1] HepG2 cells (human liver cancer-derived cell line) were seeded, and the expression levels of CYP genes were evaluated 20 days after cultivation. Compared to static culture, an increase in the expression levels of multiple CYP genes was confirmed, suggesting a state that is closer to in vivo conditions. - Cultivation Results [2] In the microtranswell of this device, vascular endothelial cells (HUVEC) were cultured on an atelocollagen sponge, resulting in tissue organization observed in some parts of the cells. *Observation by GFP fluorescence [Data provided by the Laboratory of Torisawa, Department of Microengineering, Graduate School of Engineering, Kyoto University] *We are lending out the system to those who can provide experimental data. For details on lending conditions, please contact us. *For more information, please refer to the PDF document or feel free to contact us.
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Our company has been a specialized manufacturer of over 10,000 types of solenoid valves, small pumps, and other fluid control devices for more than 60 years, providing technology related to automatic and precise control of liquids and gases. We excel in custom design tailored to the individual needs of our customers and are expanding globally. 【Customization】 As a specialist in fluid control, we flexibly respond to all product design requirements from our customers. Based on our extensive experience and fresh ideas, we actively propose various product customizations according to individual requests. We also accommodate small-scale prototypes, so please feel free to reach out. 【Miniaturization】 We are developing small and micro fluid control products, including the world's smallest class of small valves, the FV and FLV series. 【Integration】 If you provide us with your desired specifications and an overall flow diagram, we will consider the optimal selection and arrangement of components and propose integration products. <<Others>> - Established a seismic isolation factory compliant with BCP - Opened a branch in the United States in October 2012 as part of our global expansion.