Physiogenex has developed a method for NASH screening that can be implemented in three weeks. The control drugs are ezetimibe and liraglutide.
A test system has been developed by Physiogenex using a unique method (using cyclodextrin, as described later) that allows for the extremely rapid induction of NASH within just three weeks. This model uses 8-week-old male mice and measures parameters such as ALT, AST, liver weight as a percentage of body weight, total cholesterol, triglycerides, and fatty acids, all of which increase within just three weeks (at 11 weeks of age). It also shows an increase in the NAS score (indicators: steatosis, inflammation, fibrosis, ballooning, total score) and significant liver inflammation and fibrosis. Treatment with ezetimibe (5 mg/kg) for two weeks results in a decrease in plasma total cholesterol and liver fat, a reduction in gene expression related to inflammation and fibrosis, and a lower overall NAFLD score due to decreased liver inflammation. Similarly, treatment with liraglutide for two weeks also shows a reduction in body weight and liver weight, as well as a lower overall NAS score due to decreased liver inflammation. For more details, please contact us. *Inquiries can also be made through our website. We will respond promptly. (Search for "Clea Japan" at http://www.clea-japan.com/)
Inquire About This Product
basic information
Example: Group composition of 3 groups (n=10) Group 1 is the control, Groups 2 and 3 receive the test compound Oral administration once a day for 2 weeks Protocol Overview: (For details, please contact us) - Acclimatization period of 5 days for 8-week-old male mice - Feeding a high-fat, cholesterol, and cholic acid diet (HFCC diet) and providing water with added cyclodextrin starting from 9 weeks of age - One week after feeding and watering (at 10 weeks of age), random grouping based on Plasma ALT and AST. Drug administration begins (for 2 weeks) - Measurable parameters: Plasma ALT/AST, HOMA-IR, liver lipids, gene expression, pathological analysis (ORO, H&E, and Sirius Red staining), NAS scoring - Control drugs: Ezetimibe, Liraglutide *There are presentation materials regarding cyclodextrin administration. Please inquire. *You can also contact us through our website. We will respond promptly. (Search for "Clea Japan" http://www.clea-japan.com/)
Price range
Delivery Time
※Approximately 5 weeks from the exam period to the submission of the final report.
Applications/Examples of results
Main Academic Presentations APASL 2017 (oral presentation) (February 2017) Title Both ezetimibe and liraglutide improve liver inflammation in a 3-week non-alcoholic steatohepatitis mouse model
Company information
Nihon Kurea Co., Ltd. is a company primarily engaged in the production and sale of laboratory animals, the manufacture and sale of laboratory animal feed, and the manufacture and sale of laboratory animal housing equipment, tools, facilities, and devices. In response to research using laboratory animals, we have been working on the development and improvement of various types of laboratory animal feed. Currently, there are 15 different brands available. These feeds are supplied under strict management from the procurement of raw materials to formulation, manufacturing, product inspection, storage, and transportation, in accordance with the regulations of the "Nihon Kurea Co., Ltd. Business Standards for Management Criteria Related to Laboratory Animal Feed," with the aim of maintaining consistently stable quality and improving reliability.