This is a high-resolution C4 column suitable for LC/MS analysis of intact proteins (top-down proteomics).
This is a monolithic silica capillary column with a hierarchical porous structure, chemically bonded with low-adsorption functional groups (C4). It enables low-pressure and low-temperature analysis of intact proteins, making it suitable for LC/MS analysis under mild conditions. With high resolution, it allows for precise analysis of intact proteins and simultaneous analysis of various proteins. Please use it for research and development in the era of modality diversification, such as simultaneous LC/MS analysis of monoclonal antibodies, ADCs, and cell-expressed proteins. 【Features】 - Capable of separating intact proteins without enzymatic digestion. - Inactivation treatment on the silica surface suppresses carryover. - The column inner diameter is 0.1 mm, suitable for use with nano-flow LC. - The column is protected by a PEEK column jacket, making it easy to handle. *Unmodified monolithic silica capillary tubes (ULTRON HF-MBS) are also available. *For more details, please download the PDF or feel free to contact us. (Please also feel free to reach out if you would like a column demo.)
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basic information
【Specifications (Excerpt)】 ■Column Type: C4 ■Column Size (Length x Inner Diameter): 250 mm x 0.1 mm, 700 mm x 0.1 mm ■Purpose: Intact protein analysis, high hydrophobic compound analysis ■Protein Injection Amount (Guideline): 1–50 ng (1–50 µg/ml solution with 1µL injection, 250 mm column) ■Flow Rate Range: Recommended range 300–1000 nL/min ■pH Range: Recommended range 2.0–8.0 *Unmodified monolithic silica capillary tubes (ULTRON HF-MBS) are also available. *For more details, please download the PDF or feel free to contact us. (Please feel free to contact us if you would like a column demonstration.)
Price information
Price range: 100,000 to 200,000 yen *Please consult us.
Price range
P3
Delivery Time
Applications/Examples of results
- LC/MS analysis of intact proteins - Precise separation analysis of monoclonal antibodies (LC/MS analysis) - Simultaneous separation analysis of human extracted proteins - Simultaneous separation analysis of yeast extracted proteins - Analysis of protein post-translational modifications *For more details, please download the PDF or feel free to contact us.
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Company information
Since our founding, we have continuously responded to various needs of our customers in chemical analysis. In particular, in chromatography, we have been involved in the development and manufacturing of packing materials and packing columns since the establishment of the technology, accumulating analytical know-how. Based on our solid experience backed by history, we strongly support our customers' research and development.