Rapid and high precision! Introducing a method for simultaneously identifying seven genetic regions and typing bacteria. *Case study collection available.
At Fasmac, we offer a "Microbial Typing Service (NGS MLST Analysis Service)." "MLST analysis" is a method of typing bacteria by patterning mutations in multiple gene regions (usually more than 7 regions), providing detailed strain information that cannot be distinguished by 16S rRNA gene analysis. The "MLST analysis" using NGS identifies seven gene regions simultaneously by mapping sequence data obtained from shotgun genome sequencing to the genomes of closely related species, and it is believed to be faster and more accurate compared to traditional methods. We have added microbial typing analysis to Fasmac's next-generation sequencing analysis services. Please feel free to contact us if you would like to make an inquiry. *For more details, please refer to the PDF document or feel free to contact us.
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【Analysis Workflow】 ■Sample Sequence Reads ■Quality Filtering ■Estimation of Closely Related Species Using K-mer Distribution ■Mapping to Closely Related Species ■Identification of Gene Regions for MLST Analysis ■MLST Analysis Using MLST Database ■Creation of K-mer Phylogenetic Tree SNP Phylogenetic Tree ※Optional Analysis *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.