Introducing new amplicon analyses from next-generation sequencing! We present two approaches.
At Fasmac, we offer "Analysis of Live Microbial Community Structure." The "Community Structure Analysis using the Selective Membrane Permeable Dye EMA" utilizes the fact that EMA modifies DNA derived from dead bacteria, rendering the modified DNA unable to be amplified by PCR, allowing for the selective detection of DNA from live bacteria. Additionally, the "Community Structure Analysis using cDNA from Reverse Transcription of RNA" is a method for identifying actively functioning microorganisms by analyzing RNA in environmental samples. 【Features】 <Community Structure Analysis using Selective Membrane Permeable Dye EMA> ■ Utilizes the modification of dead bacterial DNA by EMA, which prevents PCR amplification of the modified DNA ■ Selectively detects DNA from live bacteria <Community Structure Analysis using cDNA from Reverse Transcription of RNA> ■ Analyzes RNA in environmental samples ■ Identifies actively functioning microorganisms *For more details, please refer to the PDF document or feel free to contact us.
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【Workflow】 <Community structure analysis using selective membrane-permeable dye EMA> ■ Prepare environmental samples (heat-treated, untreated) ■ EMA treatment ■ Light irradiation ■ DNA extraction ■ Creation of adapter library by EMA-PCR * For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.