Analysis of microbial communities in environmental samples such as soil: "Environment-related amplicon sequencing analysis."
Comprehensive analysis of microbial communities in a wide range of samples such as soil, activated sludge, environmental water, biofilms, culture media, and fermented foods, including DNA extraction, PCR, and data analysis.
- A service that conducts analysis of the microbiome (bacterial, archaeal, and fungal communities) in samples. - Supports various samples such as soil, water, biofilms, compost, contents of fermentation tanks, activated sludge, contents of septic tanks, fermented foods, probiotics, and spoiled foods. - Amplicon sequencing analysis using next-generation sequencers (MiSeq, Illumina). - We can handle the extraction of DNA and RNA, so you can send the samples directly to us when making a request. - The delivered data can be widely used, from R&D to academic papers. - Service highlights: - Excellent cost performance, starting from 22,000 yen per sample. - Comprehensive analysis of bacterial/archaeal microbiomes. - Analysis of sequences approximately 430 bp long for bacteria and approximately 385 bp long (paired-end) for archaea. - Analysis of fungal communities using the ITS region. - Acquisition of about 10,000 reads, allowing detection of low-abundance taxa. - Support for RNA analysis as well. - We also accept requests for analysis only from already acquired sequence data. Please feel free to contact us regarding analysis methods and other inquiries.
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basic information
【Analysis Content】 ○ DNA extraction and purification ○ PCR amplification and purification ○ Quality check of PCR products using a bioanalyzer ○ Quantification of each PCR product ○ Sequencing ○ Determination of DNA base sequences ○ Assembly of paired-end sequences using Fastq-join (Fastq-join file acquisition: MiSeq only) ○ Quality filtering (fasta file acquisition) ○ Data analysis 【Data analysis options available from two types of software】 ○ Set 1: Metagenome@KIN Database (bacteria and yeast): RDP + Technosuruga Lab Microbial Identification Database Database (fungi): RDP ○ Set 2: QIIME2 Database (bacteria and yeast): Greengenes database Database (fungi): UNITE 【Acceptable sample types】 Soil, water, biofilms, food, etc. 【Sample submission method】 Please send samples using methods that minimize changes in the microbial community, such as freezing or refrigeration. 【Others】 Additional analyses such as α and β diversity analysis, principal component analysis, and cluster analysis are also available.
Price information
(Price excludes tax) Set 1 Metagenome@KIN, Set 2 QIIME2 〇 Long lead time course (40 business days or more) Regardless of the number of samples, 22,000 yen / sample For prices regarding other short lead times, please refer to the related links.
Price range
P2
Delivery Time
※Depending on the selected course
Applications/Examples of results
- Analysis of bacterial communities and fungal layers in soil - Analysis of bacterial communities in septic tanks - Analysis of bacterial communities and fungal layers in compost - Analysis of bacterial communities and fungal layers in groundwater - Analysis of bacterial communities in biofilms - Analysis of bacterial communities and fungal layers in fermented foods - Analysis of bacterial communities and fungal layers in fermentation tanks
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Company information
We offer contract analysis services related to microorganisms. We support our customers' testing, analysis, and research with a wide range of services, including microbial identification, microbial community structure analysis, and intestinal environment analysis. We meet diverse needs in academic research, product research and development, and hygiene management.