Contracted quantitative testing of microorganisms "Environmental-related real-time PCR analysis (quantification)"
Quantification of total bacteria, ammonia-oxidizing bacteria, iron bacteria, sulfate-reducing bacteria, Anammox, nitrite-oxidizing bacteria, iron-oxidizing bacteria, Dehalococcoides, etc.
【Features】 - Contract analysis service to quantify the amount (copy number) of target microbial template DNA using our company's proprietary primer set. - Possession of many original primers capable of detecting environmental and food-related microorganisms. - Accurate quantification is possible even from trace samples. 【Overview】 Quantification of genes from specific taxonomic groups present in the sample. The amount of DNA, which is exponentially amplified, is monitored in real-time using a real-time PCR device. By comparing the DNA copy numbers of total bacteria and total archaea, it is also possible to estimate the proportion of target microbial groups in the sample. 【Acceptable Sample Examples】 Food samples such as fermented foods, activated sludge samples, septic tank samples, soil and soil-related samples such as volcanic ash, compost, fermentation samples such as culture media, water samples from seawater and lakes, contents of fermentation tanks, DNA extracts where PCR amplification has been confirmed, etc. 【Target Microbial Groups】 Many microbial groups can be detected and quantified. Please refer to the related links. Feel free to contact us regarding testing methods and detectable microbial groups. You can also check the details from the related links.
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basic information
【Specifications】 Using a real-time PCR system, we will perform PCR amplification with the specified primers and conduct absolute quantification of the DNA amount (copy number) of the target microorganism. 【Workflow】 〇 DNA extraction ↓ 〇 DNA purification ↓ 〇 PCR amplification (intercalator method) ↓ 〇 Monitoring of PCR products 【Report Contents】 Gene copy number (quantitative value) 【Required Sample Volume & Shipping Method】 - Food: 10 g or 100 mL; store and transport at food storage temperature - Activated sludge: 5–10 mL; refrigerate immediately after collection and transport refrigerated - Soil, compost, and manure: 1–5 g; refrigerate immediately after collection and transport refrigerated - Environmental water (tap water, low turbidity river water, etc.): 100 mL–1000 mL; refrigerate immediately after collection and transport refrigerated - Environmental water (industrial wastewater, sewage, etc.): 50–500 mL; refrigerate immediately after collection and transport refrigerated - DNA extracts: concentration: ≥10 ng/µL / total volume: ≥30 µL; refrigerate immediately after extraction and transport refrigerated If new primers need to be designed and synthesized, costs for design and synthesis, strain purchase, and standard preparation will be required.
Price information
(Prices are excluding tax) - For 1 sample with 1 primer: 43,000 yen/sample - For 2 samples with 2 primers: 42,000 yen/sample - For 5 samples with 2 primers: 28,000 yen/sample - For 10 samples with 2 primers: 24,000 yen/sample - <Option> Primer design: 40,000 yen/formula - <Option> Standard preparation: 99,000 yen/formula - Delivery time is 15 business days or more - Prices may vary depending on the number of samples and primers. Please contact us for more details.
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Delivery Time
※The delivery time may vary depending on the quantity, so please feel free to contact us.
Applications/Examples of results
- Quantification of microorganisms involved in soil purification such as bioremediation - Quantification of Anammox in the environment - Quantification of microorganisms in compost - Quantification of microorganisms involved in microbial corrosion - Quantification of microorganisms in activated sludge - Quantification of iron bacteria in groundwater - Quantification of microorganisms in fermentation tanks
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Company information
We offer contract analysis services related to microorganisms. We support our customers' testing, analysis, and research with a wide range of services, including microbial identification, microbial community structure analysis, and intestinal environment analysis. We meet diverse needs in academic research, product research and development, and hygiene management.