Contracted quantitative analysis of microorganisms "Biologically related real-time PCR analysis"
A contract service for quantifying the DNA of all bacteria, all archaea, all fungi, bifidobacteria, lactic acid bacteria, other intestinal bacteria, acne bacteria, periodontal bacteria, and more.
【Overview】 〇 This is a contract analysis service that quantitatively measures the DNA of bacteria, archaea, and fungi contained in samples. It can be used to compare microbial quantities between samples. 〇 The quantity of DNA that is exponentially amplified in real-time PCR for specific taxonomic groups present in the sample is monitored in real-time. 〇 For certain types of microorganisms, it is also possible to convert copy numbers to cell counts. 【Features】 〇 Quantification of microorganisms (bacteria, fungi, yeast, archaea) is possible. 〇 The absolute amount (copy number) of target microbial template DNA is estimated using our proprietary primer set. 〇 We possess many primers, including original primers for intestinal bacteria, oral bacteria, skin resident bacteria, and microorganisms in environmental and food samples. 〇 Accurate estimation is possible even from minute samples. 【Examples of Acceptable Samples】 〇 Animal feces and intestinal contents from mice, rats, pigs, cattle, horses, pets, etc. 〇 Saliva, plaque, skin swabs, vaginal swabs. 【Target Bacterial Groups】 Please refer to the related links. For other inquiries regarding testing methods or acceptable samples, please feel free to contact us.
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basic information
【Specifications】 Using a real-time PCR system, we will perform PCR amplification with the specified primers and conduct absolute quantification of the DNA amount (copy number) of the target microorganism. 【Report Contents】 Gene copy number (quantitative value) 【Required Sample Volume】 - Feces, intestinal contents, rumen fluid / 0.2 to 0.5 g - Oral contents (saliva, plaque), skin swabs, etc. / 0.2 to 1 mL - DNA extracts / Refrigerate immediately after extraction and transport refrigerated 【Sample Submission Method】 - Feces, intestinal contents, rumen fluid collected in our collection container (without preservative) or a highly airtight container → Freeze immediately after collection and transport frozen - Feces, intestinal contents, rumen fluid collected in our collection container (with preservative) → Refrigerate immediately after collection; refrigerated transport is recommended, room temperature transport is acceptable - Oral contents (saliva, plaque) collected in swabs, 1.5 mL tubes, etc. → Freeze immediately and transport frozen - Our Metaflo Keeper (skin) → Refrigerate immediately and transport refrigerated - Samples collected by swabbing a wide area with cotton swabs or cotton → Transfer to a very small amount of appropriate solution (PBS, saline, sterilized water, etc.), refrigerate immediately, and transport refrigerated
Price information
(Prices are excluding tax) - For 1 sample with 1 primer: 43,000 yen/sample - For 2 samples with 2 primers: 42,000 yen/sample - For 5 samples with 2 primers: 28,000 yen/sample - For 10 samples with 2 primers: 24,000 yen/sample - <Option> Primer design: 40,000 yen/formula - <Option> Standard production: 99,000 yen/formula - Delivery time is 15 business days or more. - Prices may vary depending on the number of samples and primers. Please contact us for more details.
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Delivery Time
※The delivery date may vary depending on the quantity, so please feel free to contact us.
Applications/Examples of results
- Quantification of intestinal bacteria - Quantification of probiotic microorganisms in feces - Quantification of oral bacteria - Quantification of Pg bacteria - Quantification of acne bacteria - Quantification of skin resident bacteria - Quantification of bifidobacteria in food
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Company information
We offer contract analysis services related to microorganisms. We support our customers' testing, analysis, and research with a wide range of services, including microbial identification, microbial community structure analysis, and intestinal environment analysis. We meet diverse needs in academic research, product research and development, and hygiene management.