We can also accept optional work! We will support the production of recombinant antibodies.
At Fasmac, we offer "Antibody Sequence Analysis." By obtaining the variable sequences of antibodies, we support the production of recombinant antibodies. We amplify the variable sequences of mouse IgG genes expressed in hybridomas using the 5' RACE method or RT-PCR, perform DNA cloning in E. coli, and conduct sequence analysis. Additionally, we can accommodate other optional tasks depending on the situation. Please feel free to consult with us. 【Analysis Details】 ■ Provided Material: Hybridoma total RNA 1μg (at least 100ng/μl) or cell pellet 1×10^6 cells ■ Deliverables: Report, sequence analysis data (Data for 10 clones each of H chain and L chain will be reported) ■ Delivery Time: 2 to 3 weeks *For more details, please refer to the PDF document or feel free to contact us.
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[Optional Work] ■ Antibodies other than mouse IgG ■ Determination of antibody class ■ NGS deep sequencing of variable regions ■ Sequence acquisition of constant regions by 3' RACE ■ Delivery of plasmids ■ Antigen binding test of recombinant antibodies *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.
![[Next-Generation Sequencing Analysis Case] Genotyping Analysis](https://image.mono.ipros.com/public/product/image/1d5/2000603363/IPROS58314770441313934737.png?w=280&h=280)
