Available for use in genome editing and in vitro DNA cleavage experiments! Offering both double-strand break and single-strand break types.
"GenomeCraft Cas9" is a Cas9 protein derived from Streptococcus pyogenes, equipped with nuclear localization signals at both the N-terminus and C-terminus. We offer two types of Cas9 proteins commonly used in genome editing via CRISPR/Cas: the double-strand cutting "Cas9 protein" and the single-strand cutting "Cas9 D10A nickase," which only cleaves the antisense strand of the target sequence (the complementary strand of PAM nGG). These can be used for genome editing through microinjection, electroporation, lipofection, and other methods, as well as for in vitro DNA cleavage experiments. 【Product Lineup】 ■ Cas9 Protein ・GE-005-S (Quantity: 100μg) ・GE-005-L (Quantity: 100μg x3) ■ Cas9 D10A Nickase ・GE-006-S (Quantity: 100μg) ・GE-006-L (Quantity: 100μg x3) *For more details, please refer to the PDF document or feel free to contact us.
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【Usage Examples】 ■Mouse ■Zebrafish *For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.
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