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  6. Next-generation sequencing analysis "Targeted amplicon analysis"

Next-generation sequencing analysis "Targeted amplicon analysis"

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last updated:Nov 12, 2021

ファスマック
ファスマック
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We offer a variety of optional services! Data is visualized as "microbial community composition graphs" and "heat maps." *Case study collection available.

"Target Amplicon Analysis" is a service that can be used reasonably by packaging it. The target regions for analysis include 16S V4, 18S V9, ITS (gITS7-ITS4), COI (mlCOIintF-HCO2198), and MiFish (12S V5), among others. We provide primers for sample preparation targeting the amplification regions free of charge, and you will send us your samples. After that, we will use QIIME and other tools to visualize the data as "microbial community composition graphs" and "heat maps." [Optional Services (Excerpt)] - Change of target region length (approximately 400-500 bp) - Library mix preparation - Rarefaction curve and principal coordinate analysis (PCoA) - OTU BLAST analysis *For more details, please refer to the PDF document or feel free to contact us.

    Contract Analysis
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Next-generation sequencing analysis "Targeted amplicon analysis"

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  • Related Link - http://fasmac.co.jp/amplicon_analysis

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【Analysis Overview】 (1) Our Company: Provides primers for sample preparation targeting the analysis area free of charge. (2) Customer: Conducts library preparation using PCR with the provided primers. (3) Customer: Purifies and measures the concentration for each sample, then sends a mixed sample in equal amounts. (4) Our Company: Performs QC work on the received samples. (5) Our Company: Conducts a 2×250 bp run on MiSeq for samples that pass QC. (6) Our Company: Visualizes data as "microbial community composition graphs" and "heat maps" using tools like QIIME. *For more details, please refer to the PDF document or feel free to contact us.

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Next-generation sequencing analysis

Next-generation sequencing analysis

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[Case Study] Target Amplicon Analysis

[Case Study] Target Amplicon Analysis

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[Case Study Collection - Free Download] Next-Generation Sequencing Analysis

[Case Study Collection - Free Download] Next-Generation Sequencing Analysis

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Fasmac Inc. "Technical Introduction Materials - New Added Value to Existing Platforms -"

Fasmac Inc. "Technical Introduction Materials - New Added Value to Existing Platforms -"

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The research results of Fasmac have been published in an international journal.

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The joint research results on environmental DNA between Fasmac and Professor Doi from Hyogo Prefectural University have been published in the international journal PLOS ONE. We believe that these research results will contribute to the improvement of the accuracy of environmental DNA analysis. We hope you find it useful for your research activities.

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ファスマック

ファスマック

Pharmaceuticals and Biotechnology

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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.

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