By utilizing next-generation sequencing, it is possible to perform genotyping at a higher resolution! *Case study collection available.
Genome editing technology is a powerful tool that enables free genetic modification, but genotype analysis (genotyping) is an important step in selecting modified individuals. By evaluating allele frequencies in the F0 generation through next-generation sequencing, it is possible to select individuals for breeding. Additionally, in genome editing experiments with cultured cells, a diverse population of cells with various mutations can be obtained from a single experiment, and the nature of the mutations varies depending on the experimental conditions. Next-generation sequencing allows for detailed evaluation of the mutations in the cell population. [Case Overview] - Genotyping of cultured cells using CrispRvariant - After extracting DNA from cultured cell lines edited with CRISPR/Cas, target amplicon sequencing was performed using Miseq - The obtained data was analyzed using CrispRvariant *For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.