We present a case that suggests PCR repetition is effective in increasing the number of fish species detected! *Case study collection available.
We would like to introduce an example analyzed using MiFish, which is used for DNA metabarcoding of fish. Water samples were collected from multiple locations in the Sagami River system and pooled in a plastic tank. To prevent DNA degradation, 1 ml/L of Osban disinfectant was added immediately after sampling. The samples were brought back at room temperature, and filtration and DNA extraction were performed in the lab. The obtained data was provided to MitoFish for homology search. As a result, it was observed that the number of fish species detected increased, suggesting that PCR repetition is effective in increasing the number of detected fish species. [Case Overview] - Filtration and DNA extraction were performed in the lab. - NGS library preparation was conducted using the extracted DNA. - Library preparation was performed in 8 repetitions. - The prepared library was sequenced using Illumina's MiSeq. - The obtained data was provided to MitoFish for homology search. *For more details, please refer to the PDF document or feel free to contact us.*
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【Service Contents】 ■ Acceptance from environmental water ■ Acceptance from Sterivex filter ■ Library preparation - 1st PCR 4 cycles - ・Up to 8 samples ・Up to 16 samples *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.