A highly reusable resin in which alkaline-resistant protein A is immobilized on cellulose particles that have significantly improved pressure resistance through a unique bridging method.
Cellufine SPA-HC is a highly reusable resin that immobilizes alkaline-resistant Protein A on porous cellulose particles, significantly improving pressure resistance through a unique crosslinking method. With proprietary particle manufacturing technology developed over 40 years of industry experience, it achieves high adsorption performance at high flow rates. Designed as a filler for actual manufacturing scales, it allows for the utilization of existing equipment while achieving reduced lead times. 【Features】 ■ Excellent dynamic binding capacity Achieves excellent dynamic adsorption even with short residence times Over 50 mg/mL excellent adsorption performance at a residence time of 2 minutes ■ High pressure resistance and liquid permeability Achieves high flow rates at low pressure through unique crosslinking technology At an operating pressure of 0.3 MPa, liquid flow is possible at a flow rate with a residence time of 2.4 minutes ■ Robust performance independent of antibody type Maintains high adsorption performance even with different monoclonal antibodies Enables robust downstream development ■ Excellent reusability Immobilizes highly alkaline-resistant recombinant Protein A Achieves excellent alkaline resistance *For more details, please refer to the PDF document or feel free to contact us.
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basic information
【Product Features】 ■ Ligand: Alkali-resistant Protein A ■ base resin: High-bridge cellulose particles ■ Average particle size: 70 µm ■ Operating flow rate: ≧ 650 cm/h (at 0.3 Mpa) Measured with an inner diameter of 30 cm and a bed height of 20 cm at pure water 24 ℃ ■ Dynamic binding capacity (DBC): ≧ 70 mg/ml (C10 with Polyclonal IgG) Measured at the 10% breakthrough point with a 6-minute residence time loading ■ Recommended CIP solution: 0.1 M NaOH ■ Operating temperature: 4 - 50 ℃ ■ Storage temperature: 2 - 8 ℃ in 20% ethanol ■ Chemical stability: No degradation in binding capacity was observed in a 1-week immersion test. 30% isopropanol, 20 – 70% ethanol, 8 M urea, 6 M guanidine hydrochloride, 0.1 M acetic acid ■ pH stability: 3-12 *For more details, please refer to the PDF document or feel free to contact us.*
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Applications/Examples of results
It can be used for the purification of antibody drugs. Cellufine is a chromatography resin made in Japan that has been adopted by Global Pharma. *For more details, please refer to the PDF document or feel free to contact us.
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Company information
Our group was founded in 1906 as Sogi Electric Co., Ltd., and since then, we have been a pioneer in Japan's chemical industry, contributing to the development of society by expanding renewable energy generated by hydropower into the field of electrochemistry and developing domestic nitrogen fertilizers. Our unchanging mission is to engage in manufacturing that harmonizes with the global environment, to solve our customers' challenges in order to realize a rich life, and to cultivate outstanding human resources who can thrive globally, continuously serving society.
