[Analysis Case] Quantitative Analysis of Sugars (Monosaccharides to Oligosaccharides) in Soft Drinks
It is possible to quantify the target substance! Here are examples of analyses using the HPLC-RID method and the HPLC-ELSD method.
The ultraviolet absorption of sugars (monosaccharides to oligosaccharides) can only be obtained around 190 to 195 nm, making detection difficult. Therefore, methods such as derivatization or using a refractive index detector (RID) or an evaporative light scattering detector (ELSD) are effective for measurement by HPLC. Here, we will introduce a case study analyzing sugars in soft drinks using RID and ELSD. For more details, please refer to the PDF download below. [Case Summary] ■ Analysis Samples - Commercial grape juice (20% juice, 100%) ■ Analysis Methods - HPLC-RID method, HPLC-ELSD method *For more details, please refer to the PDF document or feel free to contact us.
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【Summary】 ■ In the HPLC-RID method, sugars can be analyzed using isocratic analysis without changing the composition of the mobile phase, while in the HPLC-ELSD method, gradient analysis allows for the quantification of monosaccharides to oligosaccharides in a single analysis. ■ It is possible to quantify target substances by utilizing the characteristics of the detectors. *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Kaneka Techno Research Co., Ltd. was established in 1988 with the corporate philosophy of becoming a trusted partner for our customers in the fields of "analytical services" and "technical information services." As a group of professionals with foresight and advanced analytical and research techniques, we aim to propose solutions to the challenges faced by our customers and to provide high-quality technical services quickly, thereby becoming a trusted problem-solving technical service company. We will continue to strive for improvement every day. We sincerely request your continued guidance and support.