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  6. Analysis of protein aggregates by size exclusion chromatography (SEC)

Analysis of protein aggregates by size exclusion chromatography (SEC)

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last updated:Feb 10, 2022

カネカテクノリサーチ 本社、東京営業所、名古屋営業所、大阪分析センター、高砂分析センター
カネカテクノリサーチ 本社、東京営業所、名古屋営業所、大阪分析センター、高砂分析センター
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Evaluation by SEC is effective for protein quality control!

Quality control of proteins, including biopharmaceuticals, is important, particularly regarding aggregates such as dimers and oligomers, as well as degradation products (low molecular weight species), which have gained attention in recent years due to their relationship with immunogenicity. Size exclusion chromatography (SEC) is widely used as a simple method for analyzing these. Here, we present examples of analyses conducted on commercially available protein mixtures, IgG antibodies, and IgG antibodies that were aggregated through pH stress testing. *For more details, please refer to the PDF document or feel free to contact us.

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Analysis of protein aggregates by size exclusion chromatography (SEC)

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【Case Summary】 ■Analysis Samples  Commercial protein mixture (standard sample of known molecular weight components)  IgG antibodies (before and after stress testing) ■Analysis Method  Liquid chromatography method using SEC analysis column (detection wavelength: UV 280 nm)

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Analysis of protein aggregates by size exclusion chromatography (SEC)

Analysis of protein aggregates by size exclusion chromatography (SEC)

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カネカテクノリサーチ

カネカテクノリサーチ 本社、東京営業所、名古屋営業所、大阪分析センター、高砂分析センター

Testing, Analysis and Measurement

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Kaneka Techno Research Co., Ltd. was established in 1988 with the corporate philosophy of becoming a trusted partner for our customers in the fields of "analytical services" and "technical information services." As a group of professionals with foresight and advanced analytical and research techniques, we aim to propose solutions to the challenges faced by our customers and to provide high-quality technical services quickly, thereby becoming a trusted problem-solving technical service company. We will continue to strive for improvement every day. We sincerely request your continued guidance and support.

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