Analysis of protein aggregates by size exclusion chromatography (SEC)
Evaluation by SEC is effective for protein quality control!
Quality control of proteins, including biopharmaceuticals, is important, particularly regarding aggregates such as dimers and oligomers, as well as degradation products (low molecular weight species), which have gained attention in recent years due to their relationship with immunogenicity. Size exclusion chromatography (SEC) is widely used as a simple method for analyzing these. Here, we present examples of analyses conducted on commercially available protein mixtures, IgG antibodies, and IgG antibodies that were aggregated through pH stress testing. *For more details, please refer to the PDF document or feel free to contact us.
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【Case Summary】 ■Analysis Samples Commercial protein mixture (standard sample of known molecular weight components) IgG antibodies (before and after stress testing) ■Analysis Method Liquid chromatography method using SEC analysis column (detection wavelength: UV 280 nm)
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For more details, please refer to the PDF document or feel free to contact us.
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Kaneka Techno Research Co., Ltd. was established in 1988 with the corporate philosophy of becoming a trusted partner for our customers in the fields of "analytical services" and "technical information services." As a group of professionals with foresight and advanced analytical and research techniques, we aim to propose solutions to the challenges faced by our customers and to provide high-quality technical services quickly, thereby becoming a trusted problem-solving technical service company. We will continue to strive for improvement every day. We sincerely request your continued guidance and support.