[Analysis Case] Analysis of Triglycerides in Edible Oils (LC-TOFMS)
It is possible to estimate the structure and fatty acid composition of triglycerides!
One of the important components among the nutrients in food is lipids, and over 90% of the lipids contained in edible oils are composed of triglycerides (TG). TG has a structure where three molecules of fatty acids are esterified to glycerol. In recent years, due to increasing health awareness, omega-3 fatty acids (such as alpha-linolenic acid) and medium-chain fatty acids have also gained attention as components of TG. However, to clarify the structure of TG, structural analysis through mass spectrometry and analysis of the constituent fatty acids are necessary. Liquid chromatography-time-of-flight mass spectrometry (LC-TOFMS) is effective for the analysis of these TGs, and the structure and constituent fatty acids of TG can be estimated from the MS spectrum and MS/MS spectrum. Here, we present examples of analyses of commercially available edible oils. ■ Analysis Samples Five types of commercially available edible oils (rapeseed oil, sesame oil, olive oil, flaxseed oil, MCT oil) ■ Analysis Equipment SCIEX Triple TOF6600 (LC-TOFMS) ■ Analysis Method After dissolving the sample in tetrahydrofuran (THF), it was appropriately diluted with methanol for LC-TOFMS analysis. ■ Analysis Results For details, please refer to the PDF document.
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As an example of the analysis results of the sample, a total ion current chromatogram (TIC) obtained from rapeseed oil showed numerous detected peaks. It was found that these peaks were separated according to the total carbon number and the number of unsaturated bonds of the constituent fatty acids.
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For more details, please refer to the PDF document or feel free to contact us.
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Kaneka Techno Research Co., Ltd. was established in 1988 with the corporate philosophy of becoming a trusted partner for our customers in the fields of "analytical services" and "technical information services." As a group of professionals with foresight and advanced analytical and research techniques, we aim to propose solutions to the challenges faced by our customers and to provide high-quality technical services quickly, thereby becoming a trusted problem-solving technical service company. We will continue to strive for improvement every day. We sincerely request your continued guidance and support.