MitoPlate

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last updated：Nov 15, 2023

セントラル科学貿易

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High-resolution mitochondrial analysis MitoPlate Technology

New Probes for Mitochondrial Function In a single experiment, it is possible to conduct assays for 96 types of pre-configured mitochondrial functions. It allows for the observation of characteristics such as the rate of substrate metabolism, sensitivity to drugs and chemicals, and the effects of mutations in mitochondrial-related genes. 【Features】 ■ Mito plates come pre-set with 96 types of substrates ■ Using mito plates reveals the effects of substrates and inhibitors on mitochondrial function ■ Easy-to-use protocols for any type of cells, including adherent cells, suspended cells, transformed cells, and primary cells ■ Requires a low number of cells, from 20,000 to 40,000 per well ■ Tetrazolium redox dye allows for simple colorimetric analysis with a final electron acceptor ■ OmniLog measurement device cultivates plates with temperature control and automatically measures kinetic data for multiple plates (measures every 15 minutes for 50 plates and every 4 minutes for 16 plates) *For more details, please feel free to contact us.*

Related Link - http://www.cscjp.co.jp/foodsafety_d/biolog.html

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【Operating Procedure】 1: Prepare the assay mixture containing the membrane permeation treatment solution and the redox dye, and dispense it into each well using a pipette. ↓ 2: Add 2x cell suspension to each well and start the assay. ↓ 3: Set the microplate in the Omnilog (measuring device) and automatically measure the rate of purple color development over time. *For more details, please feel free to contact us.