Examples of usage such as "in vivo genome editing" and "double knockout" are introduced.
Here are examples of the use of the genome editing tool "Genome Craft." We mixed Cas9 protein with sgRNA or crRNA + tracrRNA and substrate DNA fragments, and conducted a DNA cleavage experiment. After incubating at 37°C for one hour, we performed agarose gel electrophoresis. The shift of the bands confirmed that both sgRNA and crRNA + tracrRNA exhibited good DNA cleavage activity. In addition, there are other examples of use such as "In vivo Genome Editing," "double knockout," and "gene knock-in (mouse)." [Examples of Use] ■ In vitro digestion assay ■ In vivo Genome Editing ■ Double knockout (zebrafish) ■ Gene knock-in (mouse) *For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.