Currently, it is widely used as a DNA testing method and DNA variety identification method under development!
"Fragment analysis" involves customers sending PCR products amplified using fluorescent primers, which are then assessed for fragment length through capillary electrophoresis by a sequencer. Our company conducts fragment analysis using a capillary sequencer to assist with DNA variety testing and technological development. We also aim to provide proposals tailored to our customers' needs, so please feel free to contact us. 【Features of DNA Sequencing Service】 - Analysis conducted domestically allows for detailed and prompt responses. - All data is visually checked by specialized staff. - The sequencer has undergone official maintenance by the manufacturer. - Re-electrophoresis is performed on samples expected to show improved data. *For more details, please refer to the PDF document or feel free to contact us.*
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basic information
**Sample Preparation Method** 1. Perform PCR using a fluorescently labeled primer at the 5' end of either the F side or the R side. 2. Conduct electrophoresis of the PCR reaction solution using the equipment you have. Please estimate and fill in the concentrations of each PCR product calculated from the marker concentrations on the electrophoresis image, and send it along with the PCR reaction solution. 3. Send 10μl of the PCR product in its original solution. Please ensure that the solution is protected from fluorescence with aluminum foil or similar materials. *For more details, please refer to the PDF document or feel free to contact us.*
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Applications/Examples of results
【Analysis Examples】 ■Personal identification using the Identifiler kit ■Variety identification of Japanese pears through microsatellite analysis ■AFLP analysis using GeneMapper *For more details, please refer to the PDF document or feel free to contact us.
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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.
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