This is a series of reading materials for technical tweets.
When outsourcing analysis, there may be concerns about the quality of RNA depending on the sample. Please read this as a hint for such situations.
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basic information
I created a reading material regarding RNA samples used in RNA-seq and CAGE-seq from the perspective of degradation. Contents: - What is FFPE? - Points where RNA degradation occurs in FFPE - RNase - RIN value: a 10-point scale indicating the degree of RNA degradation - RNA QC standards Please feel free to take a look.
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Applications/Examples of results
This is a reading material assuming the use of FFPE as a sample for RNA-seq analysis.
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Company information
Danaform Inc. is a company established in 1998, based on the RIKEN Venture System of the National Research and Development Agency RIKEN, with Yoshihide Hayasaki from RIKEN (who has been the representative director of Danaform Inc. since 2021) at its core. The company is actively engaged in gene analysis and genetic testing, focusing on technologies co-developed by Danaform and RIKEN, including contract analysis using next-generation sequencers such as CAGE-seq and RNA-seq, the sale of fluorescent reagents Eprobe capable of SNP detection, and the sale of various clones, boasting a significant inventory. Moving forward, we aim to refine our proprietary technologies and strive for higher quality research and manufacturing as experts in the field of genetics, with the hope of contributing to people's health.