DNA production without using E. coli is now possible! This can dramatically reduce the cost and time required for DNA manufacturing.
*Looking for partner companies for practical application* The "large-capacity PCR" developed by Yamaguchi University and a kamaboko manufacturing equipment company in Yamaguchi Prefecture can produce DNA in quantities more than tens of thousands of times that of conventional PCR devices used for testing, in a short time, replacing the current mainstream method of DNA production through E. coli culture. Through PCR synthesis, it is possible to synthesize DNA that does not contain unnecessary replication origins or drug resistance genes for vaccines. Additionally, because PCR-synthesized DNA is produced through enzyme reactions, the purification process can be simplified, and there will be no E. coli components when crushing and extracting E. coli plasmids. [Device Configuration] - Large-capacity dedicated thermal cycler - Large-capacity dedicated reaction liquid bag - Large-capacity dedicated reaction liquid (heat-resistant polymerase, etc.)
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Instead of the currently mainstream DNA production using E. coli culture, large amounts of DNA can be produced quickly using clean PCR. 【Device Configuration】 - High-capacity dedicated thermal cycler - High-capacity dedicated reaction liquid bags - High-capacity dedicated reaction liquid (heat-resistant polymerase, etc.) 【Breakthrough Technology】 - For large-scale PCR, two technologies are required: a device that can accurately control the temperature cycles of a large volume (e.g., 200 mL) of reaction liquid, and a reaction liquid that can perform PCR even with slow temperature changes. - Researchers from Yamaguchi University and a local kamaboko manufacturing equipment company have collaborated for many years to achieve the best match through improvements to the PCR device and adjustments to the reaction liquid. - As a result, it has become possible to conduct PCR with 200 mL of reaction liquid at once. - For the reaction liquid, which tends to be costly in PCR, a low-cost reaction liquid suitable for large-scale PCR has been developed. Patent Application No. 2017-034496 "Thermal Cycler" Patent Application No. 2020-202035 "Nucleic Acid Amplification Method and Thermal Cycler"
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【Application Areas】 - DNA drug manufacturing - DNA vaccine manufacturing - DNA reagent manufacturing - Manufacturing of products containing DNA
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Yamaguchi TLO Co., Ltd. is a TLO approved by the Ministry of Education, Culture, Sports, Science and Technology and the Ministry of Economy, Trade and Industry based on the Act on the Promotion of Technology Transfer from Universities, established in November 1999 with investments from 50 faculty members of Yamaguchi University (capital of 4 million yen) before incorporation. It is a liaison-type technology transfer organization that bridges people. We return the research results from the university to society in the form of technology transfer and other means. Additionally, we are actively recruiting member companies to prioritize the disclosure of invention information and contribute to the development of the local economy through these efforts.