[Analysis Case] Characterization of Glycosylated Proteins (LC-TOFMS)

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The LC-TOFMS (Liquid Chromatography Time-of-Flight Mass Spectrometry) method is useful for the characterization analysis of proteins (such as molecular weight analysis, peptide mapping, and glycan analysis). We will introduce a case study of the analysis and examination of the glycan (glycoform and glycan attachment sites) of IgG antibodies using the LC-TOFMS method. ■Analysis Sample: IgG Antibody ■Analysis Method: LC-TOFMS ■Analysis Results - Glycoform Analysis By analyzing the molecular weight, it is possible to estimate the glycoforms of glycoproteins. LC-TOFMS measurement of the IgG antibody revealed the presence of five different glycoforms. - Glycan Attachment Site Analysis The IgG antibody was fragmented through enzymatic treatment and measured by LC-TOFMS. The analysis of the measurement results using dedicated software showed that a N-linked glycan (G0F, G1F, G2F) was attached to the asparagine of the peptide fragment with the amino acid sequence EEQFNSTFR. *For detailed results, please refer to the PDF document.

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