[Analysis Case] Qualitative Analysis of Medium Components (LC-TOFMS)
It is possible to identify components that significantly contribute to the characteristics of the culture medium and the results of the cultivation through qualitative analysis using non-target analysis, inter-sample comparison, and principal component analysis.
In many studies using cultured cells, the qualitative and quantitative analysis of components contained in culture media and culture supernatants has gained attention and is increasingly being utilized in recent years. Understanding the components present in the media provides valuable information for improving the efficiency of cell culture. Here, we present a non-target analysis of media components using liquid chromatography-time-of-flight mass spectrometry (LC-TOFMS) through library searches, using commercially available media as examples, along with comparisons between samples and principal component analysis of the results. [Analysis Samples] Commercially available media A, B, and C [Analysis Equipment] LC-TOFMS *For detailed results, please refer to the PDF document. Qualitative analysis through non-target analysis of media components, along with comparisons between samples and principal component analysis of the results, allows for the identification of components that significantly contribute to the characteristics of the media and the outcomes of the culture. This information is expected to be useful for enhancing the results of cell culture.
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Kaneka Techno Research Co., Ltd. was established in 1988 with the corporate philosophy of becoming a trusted partner for our customers in the fields of "analytical services" and "technical information services." As a group of professionals with foresight and advanced analytical and research techniques, we aim to propose solutions to the challenges faced by our customers and to provide high-quality technical services quickly, thereby becoming a trusted problem-solving technical service company. We will continue to strive for improvement every day. We sincerely request your continued guidance and support.