There are successful examples with cells that have difficulties in cultivation! We will propose a method that suits your objectives.
We would like to introduce the "Gene-Modified Cell Line Production Service" offered by Unitec Co., Ltd. We carry out the production of cell lines with knockout or knock-in of target genes in cancer cell lines and iPS-derived cells. Our researchers will accompany you or meet with you online to propose methods that suit your objectives. We produce gene-modified cells from various cell types, including HEK293 cells, liver cancer-derived cell lines, lymphoblastoid cell lines, and osteoblast cell lines. 【Features】 ■ Researchers accompany you or meet with you online for direct discussions ■ Production of gene-modified cells from various cell types ■ Successful cases with cells that have difficulties in culture, such as those that cannot undergo limiting dilution ■ Possibility of pharmacological efficacy testing and large-scale stock *For more details, please refer to the PDF document or feel free to contact us.
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basic information
【Knockout Cell Line Production Process】 ■ Creation of test plan ■ Mycoplasma testing ■ Cell culture ■ Confirmation of target gene sequence ■ Vector construction ■ Transfection ■ Primary screening ■ Secondary screening (confirmation of protein expression) ■ Tertiary screening (genotyping) ■ Quaternary screening (confirmation of non-insertion of editing vector) ■ Scale-up culture and storage ■ Mycoplasma testing ■ Creation of test report ■ Delivery * For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Our company provides research support through services related to iPS cell contracts, genetically modified mice, animal experimentation contracts, cell-related contracts, protein expression purification and antibody production, and virus production. Please feel free to consult us if you have any requests.