Production of KI mice, it is possible to create KI mice at specific genetic loci!
We would like to introduce the "Knock-in Mouse Production" offered by Unitec Co., Ltd. It is possible to create disease model mice by introducing disease-causing SNPs into mice. We can produce mice for in vivo localization and dynamics visualization of target proteins, as well as mice that express human target proteins within their bodies. As additional options, we offer increased targeting vector supply and the production of linked embryos (200 units). We also accept requests for the production of transgenic mice. 【KI Mouse Production Process (Excerpt)】 ■ Design and construction of targeting vectors ■ Establishment of homologous recombination ES cells (derived from C57BL/6J) ■ Production of chimeric mice ■ Production of F1 heterozygous mice ■ Production of Neo sequence removal mice *For more details, please refer to the PDF document or feel free to contact us.
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[Tg Mouse Production Process] ■ Construction of vectors for transgenic mouse production ■ Cutting and purification ■ Injection of 200 fertilized eggs (Production of F0 mice) ■ Genotyping PCR of F0 mice ■ Production of F1 mice ■ Genotyping PCR of F1 mice ■ Mouse microbiological examination ■ Mouse transportation *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Our company provides research support through services related to iPS cell contracts, genetically modified mice, animal experimentation contracts, cell-related contracts, protein expression purification and antibody production, and virus production. Please feel free to consult us if you have any requests.