Assists in the separation of PBMCs from blood using a density gradient medium! Prevents mixing of blood and density gradient medium.
We would like to introduce "Leukosep," handled by Grainer Japan, Inc. This product is a specialized tube designed to assist in the separation of PBMCs from blood using a density gradient medium, with an HDPE filter that prevents the mixing of blood and the density gradient medium. Additionally, during the decantation process for recovering PBMCs, the filter can prevent the backflow of red blood cells. 【Features】 - Specialized tube for assisting in the separation of PBMCs from blood using a density gradient medium - HDPE filter prevents the mixing of blood and the density gradient medium - The filter prevents the backflow of red blood cells during the decantation process for recovering PBMCs For more details, please refer to the catalog below or contact us.
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【Instructions for Use】 ■ Warm the density liquid to room temperature while in a light-blocking state. ■ Add the density liquid to the Leucosep. 3ml for 163xxx use / 15ml for 227xxx use. ■ Tighten the screw cap and centrifuge at 1000xg for 10 minutes (at room temperature). The density liquid will move under the porous barrier. ■ When using pre-filled products with density liquid (163288-013 and 227288-013), the above steps can be omitted. In that case, please warm the entire tube to room temperature before use. ■ This completes the preparation for adding anticoagulated blood or bone marrow. Diluting the sample with buffer is not mandatory, but it may improve separation results. Recommended ratio of density liquid to sample: 1:2 (when using blood) / 1:4 (when using bone marrow). *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Greiner Bio-One is a company based in Germany and Austria that operates globally. We specialize in the development, manufacturing, and distribution of high-quality plastic products for laboratories, serving as a technology partner for hospitals, research institutes, universities, research organizations, diagnostics, pharmaceuticals, and the biotechnology industry. With seven production sites and a vast number of sales partners, we operate in over 100 countries.