No special equipment or culture medium is required! Spheroid formation can be achieved easily and in a short time.
"Magnetic3D" is a kit designed to attach nanoshuttles (particles composed of iron oxide, gold, and poly-L-lysine) to the cell surface and form spheroids using magnetic force. It allows for the formation of spheroids easily and quickly without the need for special equipment or media. Since it is cultured on low-adhesion surface cell repellent, it can be compared with spheroids that do not use magnetic beads. 【Features】 ■ Usable for cells that have difficulty forming spheroids under normal culture conditions ■ One vial of nanoshuttle can process cells equivalent to one 75cm² flask ■ No effects or toxicity to cells have been confirmed ■ Spheroid formation is rapid and can be utilized with various cell types ■ Cultured on low-adhesion surface cell repellent *For more details, please refer to the PDF materials or feel free to contact us.
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basic information
【Procedure】 1. After adding the nano shuttle to the culture medium, incubate for 6 hours to overnight. 2. Collect the cells after incubation using trypsin. The collected cells are seeded in a culture vessel that has been treated to prevent cell adhesion (Cell-Repellent products), with magnets placed on the top for 35mm dishes/6-well/24-well plates and on the bottom for 96-well/384-well plates. 3. Spheroids will begin to form in as little as 15 minutes to 1 hour. Continue culturing while keeping the magnets in place. *For more details, please refer to the PDF document or feel free to contact us.
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Applications/Examples of results
【Applications】 ■ Compound screening and toxicity testing ■ Wound healing assays ■ Organoid research ■ Stem cell research ■ Co-culture, and various other research applications *For more details, please refer to the PDF document or feel free to contact us.
Company information
Greiner Bio-One is a company based in Germany and Austria that operates globally. We specialize in the development, manufacturing, and distribution of high-quality plastic products for laboratories, serving as a technology partner for hospitals, research institutes, universities, research organizations, diagnostics, pharmaceuticals, and the biotechnology industry. With seven production sites and a vast number of sales partners, we operate in over 100 countries.
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