JIS K 6400-9 (Antibacterial Testing of Soft Foam Materials)
This is an antibacterial testing method used for soft foam materials with a continuous bubble structure (such as sponge products). In addition to this standard, there is also the SIAA shake method, which can be applied to specimens with similar properties.
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basic information
- Necessary information for conducting the test To provide a smooth estimate, it would be helpful to receive the following information: - Number of types of specimens - Whether there are any specimens prepared that correspond to unprocessed test pieces - Required deliverables* (if there are any besides the Japanese report) - Test microorganism species (if you wish to conduct the test with a species different from the specified one) *Unless there are specific requests, the report will only be in Japanese (including testing methods and results (results of determining test validity conditions, viable cell counts after culture, antimicrobial activity values), without images).
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Applications/Examples of results
**Testing Method** 1. **Preparation of Samples** Place three sets of samples (antibacterial processed test pieces and unprocessed test pieces) into sterilized containers. 2. **Preparation of Test Bacterial Solution** According to the specifications, prepare using 1/500 nutrient medium. Nutritional conditions may be altered depending on the testing purpose. 3. **Inoculation of Test Bacterial Solution** Inoculate 10 mL of the test bacterial solution into the samples. Use a Conrady rod or similar tool to remove air from inside the samples while allowing the test bacterial solution to permeate almost uniformly throughout the samples. Prepare a control group where only the test bacterial solution is inoculated without any samples. 4. **Cultivation** Incubate at 35±1°C, with an amplitude of 30±5 mm, at 150±10 rpm for 24±1 hours. 5. **Measurement of Viable Bacteria Count** For the control group, measure the viable bacteria count immediately after inoculation and 24 hours after cultivation. For the sample test group, measure the viable bacteria count 24 hours after cultivation. 6. **Evaluation** Compare the logarithmic values of the viable bacteria count of the antibacterial processed test pieces and unprocessed test pieces 24 hours after cultivation, and calculate the antibacterial activity value.
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At our center, we not only conduct tests as specified by JIS and other regulations, but we also propose testing methods that suit the purpose of the tests. We capture the entire problem for problem-solving and provide practical advice.
