Method for testing the antibacterial activity of kitchen synthetic detergents and soaps on sponges.

basic information

This is the testing method of the Detergent and Soap Fair Trade Council. It involves measuring the number of viable bacteria on a test piece after exposing it to bacteria and the sample, in order to investigate the disinfection effect. The features are as follows: 1. The test piece is used. Bacteria and the sample are brought into contact through a sponge. There is also a method of disinfecting liquid agents that does not use a test piece, which is the most basic method. 2. Nutrients are added to the bacterial solution. Nutrient media is added to the bacterial solution as a model soiling substance. When there is dirt, the effect on the bacteria of the sample tends to be less pronounced compared to when there is no dirt. *The Japanese Pharmacopoeia "Hard Surface Carrier Method" involves inoculating a bacterial solution without added nutrients onto a test piece (approximately 5 cm x 5 cm, with no specified material) and measuring the number of viable bacteria on the test piece. If you prefer this method, please let us know.

Price information

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Delivery Time

P4

※*For more details, please contact us.

Applications/Examples of results

**Testing Method** 1. **Preparation of Test Bacterial Suspension** Prepare a suspension using purified water, nutrient medium (model soil, final concentration 1/5), and hard water (final concentration 3°dH) to achieve a concentration of 10^7 to 10^8 CFU/mL. Nutritional conditions can be modified according to the testing purpose. 2. **Sample Preparation** - **Test Sample:** The original liquid of the specimen, or the specimen (liquid or powder) prepared at the specified concentration (the assumed usage concentration of the specimen or the concentration for disinfection appeal) using a solvent (such as hard water). - **Control Sample:** 0.05% (w/v) Polysorbate 80 aqueous solution. 3. **Preparation of Test Pieces** Prepare sponge pieces (soft polyurethane foam as per JIS K 6401, thickness 30 mm, without antibacterial treatment) punched out to form cylindrical shapes with a diameter of 24 mm. 4. **Inoculation of Test Bacterial Suspension and Samples onto Test Pieces** Inoculate the test pieces with the test bacterial suspension, allowing it to soak in uniformly, then inoculate with the sample and allow it to soak in uniformly as well. 5. **Cultivation** Incubate at 25±1°C for 18 hours. 6. **Measurement of Viable Bacterial Count** Wash out the test pieces and measure the viable bacterial count. 7. **Evaluation** Compare the viable bacterial counts obtained using the test sample and the control sample in logarithmic values, and calculate the disinfection activity value.