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  6. Exosome purification on a small scale using a limit filtration device.

Exosome purification on a small scale using a limit filtration device.

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last updated:Oct 18, 2024

Cytiva
Cytiva
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Built-in pre-filter for simultaneous coarse filtration and clarification! Proposal for exosome purification using ultrafiltration!

Cytiva offers a product lineup suitable for the small-scale "exosome purification" process. We provide the "AcroPrep 24-well filter plate," which is suitable for the removal of cells and debris, as well as the "Acrodisc syringe filter" with pore sizes of 0.1 and 0.2 µm, which is suitable for the removal of microvesicles. Additionally, we have a wide range of centrifugal filtration devices for exosome purification, including "Nanosep" and "Microsep." 【Product Lineup】 ■ AcroPrep 24-well filter plate ■ Acrodisc syringe filter ■ Centrifugal filtration devices for ultrafiltration - Nanosep (<0.5 mL) - Microsep (0.5-5 mL) - Macrosep (5-20 mL) - Jumbosep (20-60 mL) *For more details, please refer to the PDF document or feel free to contact us.

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2022-12-12_11h23_58.png

Exosome purification on a small scale using a limit filtration device.

2022-12-12_11h23_58.png
2022-12-12_11h23_58.png

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【Exosome Purification Workflow (Small Scale up to 50mL)】 ■(1) Removal of Cells and Debris  ・Collect culture supernatant  ・Centrifuge to remove cells and debris ■(2) Removal of Microvesicles  ・Filter supernatant using a syringe filter  ・Use 0.1 or 0.2μm diameter ■(3) Purification of Exosomes  ・Collect the filtrate and purify by ultracentrifugation *For more details, please refer to the PDF document or feel free to contact us.

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【Purpose】 ■Streamlining the exosome purification workflow *For more details, please refer to the PDF document or feel free to contact us.

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Cytiva

Cytiva

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Cytiva and Pall Life Sciences have merged their businesses and will officially launch under the "Cytiva" brand on May 1, 2023. Under our mission of "Advancing therapies, faster," we will integrate both organizations and focus even more on supporting the development and manufacturing of better therapies for patients as a stronger global provider of technologies and services. The new Cytiva will offer a broader and deeper expertise and a rich lineup of products and services to better support various customers involved in research and the manufacturing and development of biopharmaceuticals, enabling them to deliver therapies to patients more quickly, from discovery to delivery. With a rich history spanning several hundred years, Pall Life Sciences and Cytiva, as part of the Danaher Group, will continue to unite to provide better services to our customers.

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