Neo Spectrofluorometer: Example of Fluorescence Measurement of Soy Sauce

Fluorescent reactions are influenced by the wavelength of the light source and the proportion of the target being measured in the solution! Here are examples of measurements using a spectroradiometer.

As you may know, soy sauce has a dark brown color. This is due to the browning that occurs during the manufacturing process. When it is in a container of the same color, it cannot be distinguished by visual inspection or the peak wavelength of transmitted light alone. Therefore, by using ultraviolet and blue light sources to measure transmitted light, we confirmed that a slight fluorescence could be observed. By confirming the fluorescence reaction, it is possible to detect the presence of soy sauce even in colored containers. The fluorescence reaction is a phenomenon that occurs when specific wavelengths are absorbed. These wavelengths vary depending on the substance. Additionally, not all substances exhibit fluorescence reactions; this phenomenon is observed in a limited number of substances. [Summary] ■ Light source: Peak wavelength 449nm / Wavelength range 400–500nm LED (Crius) ■ Sample: Water = 20ml + Soy sauce = 0.5ml (25%) ■ 1cm square plastic cuvette, using Neo dedicated cuvette holder *For more details, please refer to the PDF document or feel free to contact us.

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