This is a series of technical tweets and reading materials.
Introducing specific factors associated with batch effects in RNA-seq.
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basic information
It explains three main elements in detail. Batch effect 1: Differences in analysis rounds, PCR and RNA fragmentation Batch effect 2: Differences in library preparation kits Batch effect 3: Differences in cell culture batches
Price range
P2
Delivery Time
※1 month to 2 months
Applications/Examples of results
The representative of next-generation sequencing (NGS) is RNA-seq. We will introduce the factors that create biases and inter-experimental differences.
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Danaform Inc. is a company established in 1998, based on the RIKEN Venture System of the National Research and Development Agency RIKEN, with Yoshihide Hayasaki from RIKEN (who has been the representative director of Danaform Inc. since 2021) at its core. The company is actively engaged in gene analysis and genetic testing, focusing on technologies co-developed by Danaform and RIKEN, including contract analysis using next-generation sequencers such as CAGE-seq and RNA-seq, the sale of fluorescent reagents Eprobe capable of SNP detection, and the sale of various clones, boasting a significant inventory. Moving forward, we aim to refine our proprietary technologies and strive for higher quality research and manufacturing as experts in the field of genetics, with the hope of contributing to people's health.