High-accuracy enzyme 'Pho DNA Polymerase'

Capable of amplifying long-chain DNA and DNA with high GC content! A research reagent for testing that is less affected by SDS.

"Pho DNA Polymerase" is a heat-stable DNA polymerase derived from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. It possesses 3’→ 5’ exonuclease activity (proofreading activity), so the resulting PCR products have blunt ends. If necessary, they can be ligated to blunt-ended vectors after phosphorylation. Additionally, the mutation frequency is approximately 10 times lower than that of Pol I type, and due to its high fidelity of synthesis, it is suitable for PCR where accurate amplification is required. 【Features】 ■ Has 3’→ 5’ exonuclease activity (proofreading activity), resulting in blunt-ended PCR products ■ High fidelity of synthesis allows for accurate PCR ■ Capable of amplifying long-chain DNA and DNA with high GC content ■ Less affected by PCR inhibitors such as SDS ■ No enzyme inactivation observed at PCR denaturation temperatures of 92℃ to 98℃ *For more details, please refer to the related links or feel free to contact us.

Other research reagentsreagentenzyme