Capable of amplifying long-chain DNA and DNA with high GC content! A research reagent for testing that is less affected by SDS.
"Pho DNA Polymerase" is a heat-stable DNA polymerase derived from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. It possesses 3’→ 5’ exonuclease activity (proofreading activity), so the resulting PCR products have blunt ends. If necessary, they can be ligated to blunt-ended vectors after phosphorylation. Additionally, the mutation frequency is approximately 10 times lower than that of Pol I type, and due to its high fidelity of synthesis, it is suitable for PCR where accurate amplification is required. 【Features】 ■ Has 3’→ 5’ exonuclease activity (proofreading activity), resulting in blunt-ended PCR products ■ High fidelity of synthesis allows for accurate PCR ■ Capable of amplifying long-chain DNA and DNA with high GC content ■ Less affected by PCR inhibitors such as SDS ■ No enzyme inactivation observed at PCR denaturation temperatures of 92℃ to 98℃ *For more details, please refer to the related links or feel free to contact us.
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basic information
【Product Contents (Partial)】 ＜Pho DNA Polymerase (50 units)＞ ■Components ・Pho DNA Polymerase (2.5 units/μl) ・dNTP Mixture (2.5 mM each) ・10 x Pho Reaction Buffer (10 mM Mg2+) *For more details, please refer to the related links or feel free to contact us.
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Applications/Examples of results
【Usage】 ■ Long PCR ■ Cloning of GC-rich templates *For more details, please refer to the related links or feel free to contact us.
Company information
Our company is a product development-oriented enterprise that utilizes biotechnology. We develop and manufacture in vitro diagnostic pharmaceuticals (such as pregnancy tests and ovulation tests), diagnostic drugs for environmental, food, and animal and plant diseases, and reagents for genetic engineering research, using our expertise in gene and antibody technology. We hold manufacturing registration and manufacturing and sales licenses for both human and animal products, with over 30 years of experience in the manufacture of in vitro diagnostic pharmaceuticals. Additionally, we are engaged in personalized medicine, advancing research and development of diagnostic drugs using genetic polymorphism analysis and gene expression analysis.