A non-woven fabric that can isolate high-purity stem cells from small amounts of human tissues such as fat, bone marrow, and umbilical cord without using collagenase.
The 『BMK-R003』 is a substrate for separating adipose stem cells that allows for the easy and rapid isolation of high-purity stem cells from a small amount of human adipose tissue. *As part of a campaign until June 30, 2025, new purchasers can buy the stem cell separation substrate at half the regular price! *Please note that this campaign is only available for purchases made through our company website. *For more details, please check our company website. The non-woven fabric with a PE-PP core-sheath structure is coated with hydroxyapatite, and by culturing human adipose tissue, bone marrow, or umbilical cord tissue on this substrate, it is possible to isolate human mesenchymal stem cells (hMSC) that produce a rich extracellular matrix. 【Features】 ■ Enables separation of stem cells with minimal damage to the cells ■ No enzymatic treatment is required, allowing for quick preparation for culture (no collagenase used) ■ Utilizes the characteristics of stem cells to achieve high-purity separation ■ Stem cells adhered to the substrate can be detached using trypsin treatment ■ Separation is possible with a small amount of tissue ■ Sterilized and ready for immediate use ■ Retains differentiation potential and cell surface markers *Please feel free to contact us.
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basic information
【Instructions】 1. Immerse the separation substrate in a sterilized dish containing ethanol. 2. Transfer the separation substrate to another sterilized dish, add saline or PBS(-), wash it, and then remove the saline (or PBS). 3. Repeat step 2 three times, then keep the separation substrate immersed in saline (or PBS). 4. Place the separation substrate containing saline into a 6-well plate. 5. After washing the adipose tissue or liposuction material with saline (or PBS), place it on top of the separation substrate (for liposuction material, a cell strainer is required). 6. Aspirate the saline (or PBS) contained in the separation substrate using a pipette. 7. Add 4-5 mL of medium to each well, ensuring that the separation substrate is submerged in the medium. 8. Incubate at 37°C for 10-14 days. 9. When the adipose stem cells occupy about 10-20% of the separation substrate, subculture them into a flask such as T-75. (Note: Perform the work in a sterile environment such as a safety cabinet, and use tweezers, dishes, culture tools, etc. as needed.) *Please feel free to contact us.
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Applications/Examples of results
For those implementing it for the first time, we recommend the "Fat Stem Cell Separation Kit: BMK-R001,004," which comes with media and instructions as a set. *For more details, please download the PDF or feel free to contact us.
Company information
Our company is engaged in business related to cells (CELL). We focus primarily on biotechnology activities such as regenerative medicine research using stem cells, reagent sales, cell analysis, and contract testing related to cells. Please feel free to contact us if you have any inquiries.