This is a research kit that can detect extracellular vesicles (EVs) such as exosomes and microvesicles in just 45 minutes! (Co-developed with Shimadzu Corporation)
EVs are substances (vesicles) secreted from cells and are abundantly found in blood and urine. Because EVs contain various information derived from the cells they originate from, they are gaining attention in the field of bio-research. For example, EVs derived from cancer cells (malignant tumors) contain information about cancer, so the detection of EVs is expected to enable early diagnosis. Additionally, in recent years, there has been a focus on the functions of EVs derived from mesenchymal stem cells (MSCs), leading to applications in the field of regenerative medicine. Despite the expectations for EVs as materials for diagnosis and medicine, existing detection methods often involve complicated processes or require expensive equipment. Our company believes that solving these challenges will contribute to the advancement of future EVs research, and we have developed this kit as a simple and cost-effective detection method. This kit uses a blue chromogenic material called "gold nanoplate," which we developed for immunochromatographic diagnostic agents, allowing for easy evaluation of the amount of EVs in the sample based on the intensity of the detection line. The simple operation makes it recommended for those starting EVs research or for use in settings where the introduction of equipment is difficult.
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basic information
【Product Features】 1) No special equipment required! Tests completed in approximately 45 minutes! 2) Ideal for startups in EV research and simple evaluations! 【Product Overview】 1) Name: Exorapid-qIC Immunochromatography Kit for Extracellular Vesicles (CD9) 2) Number of tests possible: 33 3) Contents: - Immunochromatography test strips... 37 pieces (including spares) - Gold nanoplate labeled antibody [lyophilized]... 1 vial (equivalent to 1,200µL) - Recombinant CD9 protein (standard substance) [lyophilized]... 1 vial (105ng) - Sample diluent... 1 vial (1.2mL) - Washing solution... 1 vial (10mL) - Assay microplate 96 wells... 1 piece - Instruction manual... 1 copy 4) Detection mechanism: (a) When the sample solution containing EVs is applied to the test strip, the EVs are captured by antibodies immobilized on the test strip. (b) When the gold nanoplate labeled antibody is applied, it binds to the EVs that were immobilized on the test strip in step (a). (c) The accumulation of gold nanoplate labeled antibodies at the captured EVs allows for the visual confirmation of a blue line.
Price information
Manufacturer's suggested retail price: ¥85,000 (excluding tax) 【Listing Sites】 - Product introduction (Our company website) https://www.dnt.co.jp/technology/new-business/exorapid-qic/ - Usage instructions (Our official YouTube video) https://www.youtube.com/watch?v=vagPTBKzgmo 【Contact Information】 Dainippon Toryo Co., Ltd. Specialty Business Division Functional Materials Development Group E-MAIL: evs-support@star.dnt.co.jp
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Applications/Examples of results
1) For research purposes (reagents for EV analysis) 2) Evaluation of cell activity - Confirmation of EV secretion amount from cultured cells (direct evaluation of supernatant without serum) - Background data of EVs used in experiments 3) Quantification and management of EVs - Performance evaluation of culture media and secretion enhancers - Confirmation of temporal quantity changes 4) Direct evaluation of serum samples
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Company information
Our company has a history of over 90 years since its founding in 1929 and is a comprehensive paint manufacturer that produces and sells heavy-duty anti-corrosion paints for large bridges and various plants, building materials paints for residential siding boards, interior and exterior architectural paints, metal baked paints, paints for railway vehicles and industrial machinery, and plastic paints for automobiles and home appliances. Through the product strength and technical capabilities cultivated over this history, we have created numerous products that meet the needs of the times and have built a wealth of achievements. In recent years, we have also been actively engaged in the development of environmentally friendly products such as low-solvent paints, water-based paints, and powder coatings. Moving forward, we will continue to build business models that respond to societal changes and provide services and products that only we can offer. To achieve this, we have established the "Corrosion Protection Technology Center" at our Nasu plant and the "Coating Technology Center" at our Komaki plant with the aim of creating new value together with our customers. We always consider the guideline of "Customer First" to enhance customer satisfaction and promote coexistence and mutual prosperity, while also actively advancing environmentally conscious product development, particularly initiatives based on the SDGs (Sustainable Development Goals). As we approach our 100th anniversary, we will strive to be a company that contributes to the prosperity of society and the realization of a rich life.