Options for protein preparation separation: Presentation of technical documentation for HIC columns.
Please obtain the materials regarding the detection of impurities in monoclonal antibodies using hydrophobic interaction chromatography from "PDF Download."
In recent years, there has been an increasing demand for hydrophobic interaction chromatography (HIC) columns for the analysis of protein formulations. Unlike reverse phase liquid chromatography, which denatures proteins and effectively destroys their original structure, HIC preserves the native structure and biological activity of proteins, making it useful for downstream functional analyses such as binding and cell-level potency assays. The MAbPac HIC-10 LC column and MAbPac HIC-20 column are HIC columns with wide-pore silica particles featuring patented alkylamide groups, suitable for the separation of oxidized and deamidated variants of protein formulations. The MAbPac HIC-Butyl column is an HIC column that binds butyl groups to a hydrophilic polymer matrix at an appropriate density, which helps reduce sample adsorption to the filler and suppress carryover, making it suitable for the analysis of antibody-drug conjugates (ADCs). You can view examples of analyses using our HIC columns by downloading the PDF.
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basic information
The MAbPac HIC-10 column and MAbPac HIC-20 column are HIC columns based on wide-pore silica particles with patented alkylamide groups, featuring the following characteristics: 【Features】 ■ Appropriate selectivity for variants of protein formulations such as mAbs ■ Excellent separation of oxidized variants ■ High separation capability and efficiency ■ Compatible with both organic solvent and aqueous mobile phases ■ Robust column stability The MAbPac HIC-Butyl column is a high-separation hydrophobic interaction chromatography (HIC) column based on a polymer substrate, featuring the following characteristics: 【Features】 ■ Suitable selectivity for ADC analysis ■ High separation and high-efficiency analysis ■ Stable across a wide pH range (2 to 12) ■ Low carryover ■ Good reproducibility of column packing
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Applications/Examples of results
Separation of oxidation and deamidation of protein formulations Separation of biomacromolecules that are difficult to separate under denaturing conditions using reverse phase columns Analysis of drug-to-antibody ratio in ADCs (antibody-drug conjugates)
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Company information
Thermo Fisher Scientific K.K., the Japanese subsidiary of Thermo Fisher Scientific Incorporated, is a sales company for comprehensive system analysis instruments, bio-related equipment, and research reagents. In Japan, it has bases in Yokohama, Tokyo, Osaka, Fukuoka, and Nagoya, handling various analytical instruments including mass spectrometers, various bio-related equipment, measuring instruments, medical devices, lab software, and research reagents.