Inexpensive nuclease usable during cell lysis (with properties equivalent to Benzonase Nuclease (EMD Merck)).
It is a low-cost, highly active nuclease that can digest both DNA and RNA. It can be used for the degradation of host nucleic acids during protein production in E. coli and to reduce the viscosity of solutions containing nucleic acids.
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basic information
When producing recombinant proteins in Escherichia coli, problems such as increased viscosity occur during the cell disruption process due to host-derived nucleic acids. By adding this product, it is possible to fragment host-derived nucleic acids down to a few bases, thereby reducing viscosity.
Price range
P3
Delivery Time
P3
Applications/Examples of results
Addition to the cell disruption process in recombinant protein production using Escherichia coli.
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Company information
■Construction of protein expression plasmids and protein expression/purification ■Manufacturing and sales of non-natural amino acid incorporation protein synthesis reagents (CloverDirect series) and E. coli cell-free translation kits (RYTS Kit) ■Sales of affinity ligands and affinity resins for antibody purification ■Sales of anti-KIAA polyclonal antibodies